Heterologous Expression of Chimeric Repressor of Arabidopsis ANAC046 Delays Chlorophyll Degradation in Petunia Flowers
Heterologous Expression of Chimeric Repressor of Arabidopsis ANAC046 Delays Chlorophyll Degradation in Petunia Flowers
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DOI：
10.1007/s11105-017-1049-8
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发表时间：
2017-09
影响因子：
2.1
通讯作者：
Chihiro Oda-Yamamizo;Nobutaka Mitsuda;A. Ohmiya
中科院分区：
文献类型：
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作者：
Chihiro Oda-Yamamizo;Nobutaka Mitsuda;A. Ohmiya
我们之前已经发现转录因子ANAC 046是拟南芥叶绿素降解和衰老的正调节因子。在这项研究中，我们引入过表达（OX）或显性负（SRDX）的构建体进入矮牵牛（矮牵牛杂交）的影响，重点是引进的基因对花。OX植物的花冠肢表现出增强的叶绿素分解代谢基因水平，导致叶绿素含量低于野生型植物。在SRDX植物中，叶绿素分解代谢基因被下调，并且在花冠肢中积累了较高水平的叶绿素。野生型和转基因植株衰老相关基因的表达水平和花寿命无显著差异。在矮牵牛叶片中，ANAC046促进叶绿素降解和衰老，如先前在矮牵牛叶片中观察到的。我们的结论是，共同的或密切相关的转录因子调节叶绿素降解可能存在于叶和花，而那些参与衰老是不同的。
We have previously found that a transcription factor, ANAC046, acts as a positive regulator of chlorophyll degradation and senescence inArabidopsisleaves. In this study, we introduced overexpression (OX) or dominant negative (SRDX) constructs into petunia (Petunia hybrida) with a focus on the effect of the introduced gene on the flowers. The corolla limbs of the OX plants showed enhanced levels of chlorophyll catabolic genes resulting in lower chlorophyll content than those of wild-type plants. In the SRDX plants, chlorophyll catabolic genes were down-regulated and a higher level of chlorophyll was accumulated in the corolla limbs. There was no significant difference in the expression levels of senescence-associated genes and flower longevity between wild-type and transgenic plants. In petunia leaves, ANAC046 promoted chlorophyll degradation and senescence as previously observed inArabidopsisleaves. We conclude that common or closely related transcription factors that regulate chlorophyll degradation may exist in the leaves and flowers, whereas those involved in senescence are different between them.