Arginine and Macrophage Activation
Arginine and Macrophage Activation
复制标题
DOI：
10.1007/978-1-61779-527-5_16
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Celada, Antonio
中科院分区：
文献类型：
--
作者：
Comalada, Monica;Yeramian, Andree;Celada, Antonio
为了发挥其功能，巨噬细胞必须被Th 1型细胞因子如干扰素-γ激活，称为经典激活或M1，或者被Th 2型细胞因子如IL-4、IL-10、IL-13等激活，称为替代激活或M2。在所有这些条件下，巨噬细胞需要摄取外源性精氨酸以满足其代谢需求。取决于这种氨基酸的细胞内可用性，这些细胞的活性被差异地调节。在这方面，巨噬细胞活化需要这种氨基酸来合成蛋白质，通过经典活化产生一氧化氮，以及通过交替活化产生多胺和脯氨酸。因此，研究氨基酸系统转运蛋白的精氨酸转运可能是巨噬细胞生理反应的关键调控步骤。在本章中，我们提出了简单和直接的方法来确定精氨酸转运蛋白的mRNA表达和活性。此外，我们描述了一种直接的方法来测量精氨酸催化剂使用薄层色谱法。
In order to perform their functions, macrophages must be activated either by Th1-type cytokines, such as interferon-gamma which is called classical activation or M1, or by Th2-type cytokines, such as IL-4, IL-10, IL-13, etc. referred as alternative activation or M2. In all of these conditions, macrophages require the uptake of exogenous arginine to meet their metabolic demands. Depending on the intracellular availability of this amino acid, the activities of these cells are differentially modulated. In this regard, macrophage activation requires this amino acid for the synthesis of proteins, production of nitric oxide via classical activation, and production of polyamines and proline through alternative activation. Therefore, the study of the arginine transport for amino acid system transporters may be a key regulatory step for physiological responses in macrophages. In this chapter, we present simple and direct methods to determine the mRNA expression and activity of arginine transporters. Moreover, we describe a direct method to measure the arginine catabolism using thin-layer chromatography.