Metabolism and incorporation into glycerolipids of exogenous 18:3(n-3) and 18:3(n-6) by MDCK cells.
Metabolism and incorporation into glycerolipids of exogenous 18:3(n-3) and 18:3(n-6) by MDCK cells.
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MDCK 细胞代谢并掺入外源 18:3(n-3) 和 18:3(n-6) 甘油脂。
DOI：
10.1007/bf02536402
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发表时间：
1986
期刊：
影响因子：
1.9
通讯作者：
Schneeberger,EE
中科院分区：
文献类型：
--
作者：
Lynch,RD;Locicero,J;Schneeberger,EE
The extent to which exogenous 18∶3(n−3) and 18∶3(n−6) were desaturated and elongated and the degree to which they and their derivatives altered the unsaturation index of cell glycerolipids were compared using clone 4 MDCK cells grown in lipid- and serum-free medium. Despite differences in the degree of unsaturation of the individual polyunsaturated fatty acids produced from 18∶3(n−3) or 18∶3(n−6), the unsaturation index of phospholipids increased similarly from 0.7 in control cells grown in serum- and lipid-free medium to ca. 1.6 in those supplemented with fatty acid. The added fatty acids had little effect on cell growth. The conversion of 18∶3(n−6) to 20∶3(n−6) and 20∶4(n−6) was more rapid than that of 18∶3(n−3) to 20∶4(n−3) and 20∶5(n−3). No significant quantities of 20∶3(n−3) or 18∶4(n−3) were noted. When both 18∶3 isomers were supplied simultaneously, marked differences in the amounts of some species of n−3 and n−6 polyunsaturated fatty acids were observed. The presence of 18∶3(n−6) and/or its derivatives suppressed levels of 20∶4(n−3) and 20∶5(n−3), perhaps through inhibition of the Δ6 and Δ5 desaturases responsible for their synthesis from 18∶3(n−3). Similarly 18∶3(n−3), and/or its longer more unsaturated derivatives, diminished the formation of 20∶4(n−6) from 18∶3(n−6). No marked effect on the products derived from elongation alone were observed.
