Berberine attenuates the inflammatory response by activating the Keap1/Nrf2 signaling pathway in bovine endometrial epithelial cells
Berberine attenuates the inflammatory response by activating the Keap1/Nrf2 signaling pathway in bovine endometrial epithelial cells
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DOI：
10.1016/j.intimp.2021.107738
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发表时间：
2021-05-10
影响因子：
5.6
通讯作者：
Cao, Rongfeng
中科院分区：
文献类型：
--
作者：
Fu, Kaiqiang;Wang, Zezhi;Cao, Rongfeng
子宫内膜炎是一种常见的炎症性疾病，困扰着奶牛养殖业的快速发展。在本研究中，我们研究了小檗碱对LPS诱导的牛子宫内膜上皮细胞（bEECs）炎症反应的抗炎作用，以及Keap 1/Nrf 2信号通路在此过程中的参与。小檗碱处理显著降低bEEC中LPS诱导的CRP、IL 1 β、IL-6和TNF-α的表达水平。这些细胞中的Nrf 2信号通路也被小檗碱激活。我们进一步评估了Nrf 2激活剂和抑制剂对促炎细胞因子下调的影响。Nrf 2的激活剂显著抑制LPS诱导的这些促炎细胞因子的产生。然而，Nrf 2的抑制剂仅部分抑制小檗碱对LPS诱导的bEECs炎症反应的抗炎作用。总之，我们的研究结果表明，小檗碱发挥抗炎作用，部分通过激活Keap 1/Nrf 2信号通路。
Endometritis is a common inflammatory disease that disturbs the rapid development of dairy farming. In the present study, we investigated the anti-inflammatory effects of berberine on the LPS-induced inflammatory response in bovine endometrial epithelial cells (bEECs) and the participation of the Keap1/Nrf2 signaling pathway in this process. Berberine treatment significantly reduced the LPS-induced expression levels of CRP, IL1 beta, IL-6, and TNF-alpha in bEECs. The Nrf2 signaling pathway in these cells was also activated by berberine. We further evaluated the effects of Nrf2 activators and inhibitors on the downregulation of proinflammatory cytokines. The activator of Nrf2 significantly inhibited the production of these proinflammatory cytokines that was induced by LPS. However, an inhibitor of Nrf2 only partially inhibited the anti-inflammatory effects of berberine on the LPS-induced inflammatory response in bEECs. In conclusion, our findings suggest that berberine exerts anti-inflammatory effects partially by activating the Keap1/Nrf2 signaling pathway.