The repeated sequence CATT(A/T) is required for granulocyte-macrophage colony-stimulating factor promoter activity.
The repeated sequence CATT(A/T) is required for granulocyte-macrophage colony-stimulating factor promoter activity.
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重复序列 CATT(A/T) 是粒细胞-巨噬细胞集落刺激因子启动子活性所必需的。
DOI：
10.1128/mcb.10.11.6084-6088.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Gasson,J
中科院分区：
文献类型：
--
作者：
Nimer,S;Fraser,J;Richards,J;Lynch,M;Gasson,J
造血生长因子GM-CSF（粒细胞-巨噬细胞集落刺激因子）由活化的T淋巴细胞表达，但不由静息的T淋巴细胞表达。先前，我们将GM-CSF诱导型启动子活性定位于GM-CSF 5′侧翼序列的90 bp区域，从bp -53延伸至+37。为了更精确地鉴定T淋巴细胞中诱导型启动子活性所需的GM-CSF DNA序列，我们在含有重复序列CATT（A/T）的GM-CSF 5′侧翼序列（bp -57至-24）区域内进行了诱变。不改变重复的CATT（A/T）序列的突变不消除诱导型启动子活性，而CATT（A/T）重复序列中的任一个的突变或缺失消除T细胞系和原代人T淋巴细胞中的所有诱导型启动子活性。因此，直接重复CATT（A/T）的两个拷贝是T细胞中GM-CSF的有丝分裂原诱导表达所需的。
The hematopoietic growth factor GM-CSF (granulocyte-macrophage colony-stimulating factor) is expressed by activated but not resting T lymphocytes. Previously, we localized GM-CSF-inducible promoter activity to a 90-bp region of GM-CSF 5′-flanking sequences extending from bp -53 to +37. To more precisely identify the GM-CSF DNA sequences required for inducible promoter activity in T lymphocytes, we have performed mutagenesis within a region of GM-CSF 5′-flanking sequences (bp -57 to -24) that contains the repeated sequence CATT(A/T). Mutations that do not alter the repeated CATT(A/T) sequence do not eliminate inducible promoter activity, whereas mutation or deletion of either of the CATT(A/T) repeats eliminates all inducible promoter activity in T-cell lines and in primary human T lymphocytes. Thus, both copies of the direct repeat CATT(A/T) are required for mitogen-inducible expression of GM-CSF in T cells.