Anterior distribution of human olfactory epithelium
Anterior distribution of human olfactory epithelium
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DOI：
10.1097/00005537-200003000-00016
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发表时间：
2000-03-01
期刊：
影响因子：
2.6
通讯作者：
Kobal, G
中科院分区：
文献类型：
--
作者：
Leopold, DA;Hummel, T;Kobal, G
目的/假设：目的：通过嗅觉电图（EOG）和解剖定位的活检标本，研究人嗅上皮的功能分布。研究设计：前瞻性、非随机、调查性。研究方法：对12名健康的受过训练的志愿者（6名女性，6名男性;年龄范围，21-48岁）进行了超阈值EOG记录，使用香草醛作为刺激物，因为它只兴奋嗅觉受体神经元。采用细纤维内窥镜引导下放置的管状电极记录EOG。在常规鼻和鼻窦手术中，在15名嗅觉测试患者（7名女性，8名男性;年龄范围，22-60岁）的EOG阳性区域的前上鼻腔粘膜中获得活检标本。对活检组织进行组织学处理，并对嗅觉和神经蛋白进行染色。结果：12例受试者中有7例对EOG测试获得了有效反应。在这7例受试者中，可以确定在中鼻甲前附着点上方或下方获得EOG的9个部位。活检结果显示在同一区域有成熟的嗅觉受体神经元。结论：人类嗅上皮似乎比以前假设的更向前分布。
Objectives/Hypothesis: To functionally investigate the distribution of the olfactory epithelium in humans by means of the electro-olfactogram (EOG) and anatomically located biopsy specimens. Study Design: Prospective, nonrandomized, investigational. Methods: Supra-threshold EOG recordings were made on 12 healthy, trained volunteers (6 women, 6 men; age range, 21-48 y), Vanillin was used as the stimulus, since it exclusively excites olfactory receptor neurons. The EOG was recorded with tubular electrodes that were placed using thin-fiber endoscopic guidance. Biopsy specimens were obtained of anterosuperior nasal cavity mucosa in the same regions as the positive EOGs in 15 smell-tested patients (7 women, 8 men; age range, 22-60 y) during routine nasal and sinus surgery. This biopsied tissue was histologically processed and stained for olfactory and neural proteins. Results: Viable responses to EOG testing were obtained in 7 of 12 subjects. In these seven subjects it was possible to identify nine sites above or below the anterior middle turbinate insertion where EOGs were obtained. The biopsy results showed mature olfactory receptor neurons in this same area. Conclusions: Human olfactory epithelium appears to be distributed more anteriorly than previously assumed.