A theory for the electrophoretic separation of DNA in polymer solutions.
A theory for the electrophoretic separation of DNA in polymer solutions.
复制标题
聚合物溶液中 DNA 电泳分离的理论。
DOI：
10.1002/elps.1150191814
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Blanch,HW
中科院分区：
文献类型：
--
作者：
Sunada,WM;Blanch,HW
基于DNA与聚合物分子之间的非纠缠碰撞，提出了一个聚合物溶液中DNA毛细管电泳的数学模型。使用视频显微镜图像的DNA迁移通过聚合物溶液，我们提出了一个修改后的瞬态纠缠耦合机制的DNA分离，包括非纠缠的DNA/聚合物碰撞。我们表明，一个数学模型的基础上个人nonentangling DNA/聚合物碰撞是能够预测的DNA在低分子量聚合物的解决方案中的迁移率。我们比较模型预测的迁移率数据的范围内的浓度为羟丙基纤维素（MW 100 000）和羟乙基纤维素（MW 139 000）的溶液中的DNA分离。该模型依赖于一个拟合参数，即DNA和聚合物分子之间相互作用的时间常数，该参数基于DNA和聚合物的物理性质。
We present a mathematical model of DNA capillary electrophoresis in polymer solutions based on nonentangling collisions between DNA and polymer molecules. Using videomicroscopy images of DNA migrating through polymer solutions, we propose a modified transient entanglement coupling mechanism for DNA separations that includes nonentangling DNA/polymer collisions. We show that a mathematical model based on individual nonentangling DNA/polymer collisions is able to predict the mobilities of DNA in solutions of low molecular mass polymer. We compare the model predictions to mobility data for separations of DNA in a range of concentrations for solutions of both hydroxypropylcellulose (Mw100 000) and hydroxyethylcellulose (Mw139 000). The model relies on one fitted parameter, the time constant for the interaction between the DNA and polymer molecules, which is based on the physical properties of DNA and polymers.