Thymoquinone reduces hepatic glucose production in diabetic hamsters
Thymoquinone reduces hepatic glucose production in diabetic hamsters
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DOI：
10.1016/j.rvsc.2005.01.001
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发表时间：
2005-12-01
影响因子：
2.4
通讯作者：
Takewaki, T
中科院分区：
文献类型：
--
作者：
Fararh, KM;Shimizu, Y;Takewaki, T
本研究的目的是从肝脏葡萄糖生成的角度，阐明胸腺醌对链脲佐菌素(STZ)诱导的糖尿病仓鼠降糖作用的机制。以65 mg/kg体重的链脲佐菌素(STZ)腹腔注射诱导糖尿病模型。用百里酚治疗糖尿病4周后开始，每日剂量为50 mg/kg体重(胃测量法)。血糖和糖化血红蛋白水平根据治疗时间的不同而显著降低。用药30天后，用胶原酶分离肝细胞，测定肝葡萄糖产量。体外培养的肝细胞与糖异生前体(丙氨酸、甘油和乳酸)孵育2 h后，用百里香酚处理的金黄地鼠的葡萄糖生成量显著低于空白对照组。这项研究的结果表明，百里香酚的抗糖尿病作用至少部分是通过减少肝脏的糖异生来实现的。(C)2005爱思唯尔有限公司。保留所有权利。
The aim of this study was to elucidate the mechanisms underlying the glucose lowering effects of thymoquinone in streptozotocin (STZ)-induced diabetic hamsters in terms of hepatic glucose production. Diabetes was induced by intraperitoneal injection of 65 mg/kg body weight of STZ. Treatment with thymoquinone commenced 4 weeks after induction of diabetes at a daily dose of 50 mg/kg body weight by gastric gauge. Blood glucose and glycated hemoglobin levels were significantly reduced depending on periods of the treatment. Thirty days after commencement of thymoquinone-treatment, hepatocytes were isolated using collagenase to determine liver glucose production. Glucose production after 2 h incubation of the isolated hepatocytes with gluconeogenic precursors (alanine, glycerol and lactate) was significantly lower in hamsters treated with thymoquinone as compared to that in vehicle controls. The results of this study demonstrate that the antidiabetic action of thymoquinone is at least partially mediated through a decrease in hepatic gluconeogenesis. (c) 2005 Elsevier Ltd. All rights reserved.