Photoperiod and testosterone regulate androgen receptor immunostaining in the Siberian hamster brain.
Photoperiod and testosterone regulate androgen receptor immunostaining in the Siberian hamster brain.
复制标题
光周期和睾酮调节西伯利亚仓鼠大脑中的雄激素受体免疫染色。
DOI：
10.1095/biolreprod.102.010900
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Jetton,AmyE
中科院分区：
文献类型：
--
作者：
Bittman,EricL;Ehrlich,DavidA;Ogdahl,JustyneL;Jetton,AmyE
Day length regulates the effects of gonadal steroids on gonadotropin secretion and behavior in seasonal breeders. To determine whether this influence of photoperiod results from changes in androgen receptor expression in Siberian hamster brain regions that regulate neuroendocrine function, androgen receptor immunostaining was examined in castrated animals given either no androgen replacement or one of three doses of testosterone (T) resulting in physiological serum concentrations. Half of the animals were housed under inhibitory photoperiod conditions, and immunostaining was quantified 11 days later. Measurement of serum gonadotropin and prolactin concentrations confirmed that androgen exerted graded effects on pituitary function but that the animals were killed before photoperiodic influences had fully developed. T significantly increased the numbers of androgen receptor-immunoreactive cells in every brain region examined. Photoperiod exerted no significant influence on androgen receptor-immunoreactive cell number in the arcuate nucleus, bed nucleus of the stria terminalis (BNST), medial preoptic nucleus, or in medial amygdala. An interaction between T and photoperiod was observed in the BNST and in the rostral and middle portions of the arcuate nucleus. Although increasing concentrations of T resulted in more intense cellular immunostaining in the BNST and arcuate, this effect was not influenced by day length. These results indicate that relatively short-duration (11 days) exposure to inhibitory photoperiod triggers localized and regionally specific changes in androgen receptor expression.
