SUBSTRATE PREPARATION FOR RELIABLE IMAGING OF DNA-MOLECULES WITH THE SCANNING FORCE MICROSCOPE
SUBSTRATE PREPARATION FOR RELIABLE IMAGING OF DNA-MOLECULES WITH THE SCANNING FORCE MICROSCOPE
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DOI：
10.1016/0304-3991(92)90430-r
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发表时间：
1992-07-01
期刊：
影响因子：
2.2
通讯作者：
BUSTAMANTE, C
中科院分区：
文献类型：
--
作者：
VESENKA, J;GUTHOLD, M;BUSTAMANTE, C
提出了一种用扫描力显微镜（SFM）成像环状DNA分子的简单底物制备方法。这些生物分子被吸附在云母上，云母浸泡在醋酸镁中，经过超声处理并发光释放。在样品损坏发生之前，手写笔-样品可能承受的力取决于环境的相对湿度。圆形DNA分子的图像通常是用经过曲率半径R(c)约为10nm [D]的电子束特殊修饰的尖端获得的。陈志忠，《海洋科学》（1992），第3期。这些被吸附的生物分子的分辨率由R(c)决定。在更高的力下，单个环状DNA分子可以用SFM触控笔操作。将讨论开发更锐利探针的策略。
A simple method of substrate preparation for imaging circular DNA molecules with the scanning force microscope (SFM) is presented. These biomolecules are adsorbed onto mica that has been soaked in magnesium acetate, sonicated and glow-discharged. The stylus-sample forces that may be endured before sample damage occurs depends on the ambient relative humidity. Images of circular DNA molecules have been obtained routinely using tips specially modified by an electron beam with a radius of curvature, R(c), of about 10 nm [D. Keller and C. Chih-Chung, Surf Sci. 268 (1992) 333]. The resolution of these adsorbed biomolecules is determined by the R(c). At higher forces individual circular DNA molecules can be manipulated with the SFM stylus. Strategies to develop still sharper probes will be discussed.