Structural Significance of Lipid Diversity as Studied by Small Angle Neutron and X-ray Scattering.
Structural Significance of Lipid Diversity as Studied by Small Angle Neutron and X-ray Scattering.
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DOI：
10.3390/membranes5030454
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发表时间：
2015-09-21
期刊：
影响因子：
4.2
通讯作者：
Katsaras J
中科院分区：
文献类型：
--
作者：
Kučerka N;Heberle FA;Pan J;Katsaras J
我们回顾了近年来在快速发展的膜生物物理学领域的发展，重点是由不同的散射技术，即中子和X射线散射确定的单脂双层的结构特性。精确的脂质结构特性的需要强调的是，有时在文献中发现的相互矛盾的结果，即使在大多数研究的脂质双层的情况下。越来越多的，准确和详细的结构模型需要更多的实验数据，如对比度不同的中子散射和X射线散射实验，与分子动力学模拟联合细化。这种实验和计算方法产生了稳健的双层结构参数，这些参数使得能够洞察例如集体膜特性及其组分（例如，烃链长度和不饱和度以及脂质头基组成）。从诸如这些的模型研究中，人们能够更好地理解如何通过平衡脂质的不同部分（例如，酰基链、头基、主链等）。
We review recent developments in the rapidly growing field of membrane biophysics, with a focus on the structural properties of single lipid bilayers determined by different scattering techniques, namely neutron and X-ray scattering. The need for accurate lipid structural properties is emphasized by the sometimes conflicting results found in the literature, even in the case of the most studied lipid bilayers. Increasingly, accurate and detailed structural models require more experimental data, such as those from contrast varied neutron scattering and X-ray scattering experiments that are jointly refined with molecular dynamics simulations. This experimental and computational approach produces robust bilayer structural parameters that enable insights, for example, into the interplay between collective membrane properties and its components (e.g., hydrocarbon chain length and unsaturation, and lipid headgroup composition). From model studies such as these, one is better able to appreciate how a real biological membrane can be tuned by balancing the contributions from the lipid’s different moieties (e.g., acyl chains, headgroups, backbones, etc.).