Establihment of an experimental liver metastasis model by intraportal injection of a newly derived human pancreatic cancer cell line (KLM-1)
Establihment of an experimental liver metastasis model by intraportal injection of a newly derived human pancreatic cancer cell line (KLM-1)
复制标题
通过门静脉内注射新衍生的人胰腺癌细胞系（KLM-1）建立实验性肝转移模型
DOI：
--
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
S. Matsuno
中科院分区：
文献类型：
--
作者：
Y. Kimura;M. Kobari;M. Sunamura;M. Kimura;H. Shimamura;S. Matsuno
SummaryConclusionIt is suggested that this liver metastasis model formed by a highly metastatic variant (KLM-1) is valuable for the study of the liver metastatic processes of human pancreatic cancer.BackgroundLiver metastasis in the early postoperative period is one of the causes for the poor prognosis of patients with resected pancreatic cancer. Therefore, it is necessary to establish an experimental model to study the mechanisms of liver metastasis in pancreatic cancer.MethodsHuman pancreatic cancer cell lines (PK-1, PK-9, and KLM-1) were injected into the portal vein of nude mice with or without pretreatment with antiasialo GM1, and colonies of liver metastases were counted for comparison of metastatic ability of these cell lines. Biological and histopathological characteristics of the highly liver metastatic cell line (KLM-1) were compared with its parent cell line (PK-1).ResultsPK-1 cells and PK-9 cells rarely formed liver metastasis foci, but pretreatment with antiasialo GM1 promoted liver metastasis. KLM-1 cells formed liver metastases at the rate of 70% even without antiasialo GM1 pretreatment. KLM-1 cells had such biological characteristics as short doubling time, short lag phase, and resistance to NK cytotoxicity. After intraportal injection of125I-labeled KLM-1 cells, radioactivitiy as well as micrometastases were detected in the liver at 72 h.
影响因子：
11.2
作者：
K. Morikawa;S. Walker;J. Jessup;I. Fidler
通讯作者：
K. Morikawa;S. Walker;J. Jessup;I. Fidler
Lewis肺癌变异体的粘附性和形态影响其对自然杀伤细胞溶解的敏感性及其转移能力。
DOI：
--
发表时间：
1987
期刊：
Invasion & metastasis
影响因子：
--
作者：
Young,MR;Duffie,GP;Newby,M
通讯作者：
Newby,M