Testis-specific expression of the mouse histone gene H1t is regulated by several promoter elements
Testis-specific expression of the mouse histone gene H1t is regulated by several promoter elements
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DOI：
10.1095/biolreprod57.6.1375
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发表时间：
1997-12-01
影响因子：
3.6
通讯作者：
Doenecke, D
中科院分区：
文献类型：
--
作者：
Drabent, B;Doenecke, D
睾丸特异性组蛋白基因H1 t仅在粗线期精母细胞阶段的哺乳动物睾丸中表达。在小鼠睾丸原代培养细胞中检测了小鼠H1 t基因的组织特异性调节，所述小鼠睾丸原代培养细胞具有由融合到氯霉素乙酰转移酶或萤火虫荧光素酶报告基因的H1 t启动子元件组成的基因构建体。我们的实验表明，小鼠H1 t基因的表达增强了一个保守的H1组蛋白基因特异性TG盒452碱基对上游的转录起始位点。H1 t基因的转录似乎被-1999和-1506之间的序列降低。H1盒在小鼠H1 t基因的表达中没有显示出调节作用。从小鼠睾丸中提取的核蛋白的结合，这些共识元素的电泳迁移率变化测定与小鼠睾丸核蛋白和标记的寡核苷酸含有的上游TG盒序列或两个睾丸特异性元件的H1 t基因。
The testis-specific histone gene H1t is expressed only in mammalian testis at the stage of pachytene spermatocytes. The tissue-specific regulation of the mouse H1t gene was examined in mouse testicular primary culture cells with gene constructs consisting of H1t promoter elements fused to the chloramphenicol acetyltransferase or the firefly luciferase reporter gene. Our experiments demonstrate that expression of the mouse H1t gene is enhanced by a conserved H1 histone gene-specific TG box 452 base pairs upstream of the transcription start site. The transcription of the H1t gene appears to be reduced by sequences between -1999 and -1506. No regulatory effect could be shown for the H1 box in the expression of the mouse H1t gene. Binding of nuclear protein extracted from mouse testis to these consensus elements was shown by electrophoretic mobility-shift assays with mouse testicular nuclear proteins and labeled oligonucleotides containing the upstream TG box sequences or the two testis-specific elements of the H1t gene.