Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).
Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).
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雌二醇-17β和黄体酮之间相互作用对克隆乳腺肿瘤细胞（MCF-7和T47D）增殖的影响。
DOI：
10.1002/jcp.1041240305
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发表时间：
1985
影响因子：
5.6
通讯作者：
Sonnenschein,C
中科院分区：
文献类型：
--
作者：
Schatz,RW;Soto,AM;Sonnenschein,C
我们之前曾报道过，增加活性炭-葡聚糖剥离的女性人血清（CDFHS）浓度可抑制克隆的MCF-7和T47 D人乳腺肿瘤细胞的增殖。在培养基中加入3 × 10− 11 M雌二醇-17 β，可恢复最大增殖率。这些观察结果表明，T47 D和MCF-7细胞的增殖受到血液传播抑制剂的调节，其作用被雌激素中和。在本报告中，我们探讨了孕酮改变雌激素反应的可能性。MCF-7细胞在含有2- 40%CDFHS的DME中生长。孕酮浓度为3 × 10− 7 M至3 × 10− 12 M时，对在存在或不存在雌二醇-17 β的情况下培养的MCF-7或T47 D细胞的产量没有影响。
We have previously reported that the proliferation of cloned MCF‐7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal‐dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 × 10−11M estradiol‐17β to the culture media. These observations suggest that the proliferation of T47D and MCF‐7 cells is regulated by a blood‐borne inhibitor whose effects are neutralized by estrogens. In the present report we explore the possibility that progesterone alters the estrogenic response. MCF‐7 cells were grown in DME containing 2–40% CDFHS. Progesterone, at 3 × 10−7M to 3 × 10−12M, had no effect on the yield of MCF‐7 or T47D cells that were cultured in the presence or absence of estradiol‐17β.