Correlation between genetic and biological aspects in primary non-metastatic breast cancers and corresponding synchronous axillary lymph node metastasis
Correlation between genetic and biological aspects in primary non-metastatic breast cancers and corresponding synchronous axillary lymph node metastasis
复制标题
DOI：
10.1007/s10549-006-9300-2
复制
发表时间：
2007-03-01
影响因子：
3.8
通讯作者：
Mingazzini, Pietro L.
中科院分区：
文献类型：
--
作者：
D'Andrea, Mario R.;Limiti, Maria R.;Mingazzini, Pietro L.
本研究调查了一组明确的细胞增殖、分化和凋亡标志物的表达水平的变化（如果有的话），这些标志物在原发性乳腺肿瘤和来自具有可比疾病状态的患者群体的相应同步淋巴结转移之间，在临床特征和自然史方面存在变化。本研究纳入了 90 例纯浸润性导管癌，涉及 10 个或更多腋窝淋巴结，且无远处转移证据。使用标准方法通过免疫组织化学评估原发肿瘤和相应的转移淋巴结组织标本中细胞周期蛋白B1、MMP1金属蛋白酶、ICAM-1、RARβ、Ki67、ER、PgR、p53、bcl-2和c-erbB2的表达。双变量 Pearson 相关分析证明原发淋巴结和匹配的相应转移淋巴结之间存在密切关系。即使根据染色表达的每个标记物截止水平将染色结果分类为阳性/阴性，仍保持高度相关性。我们报告了对一组明确的浸润性导管癌患者的生物决定因素进行的最广泛的免疫组织化学分析，这些患者患有 10 个或更多腋窝淋巴结且无远处转移。我们发现，就本研究中研究的所有 10 种标记物的表达而言，原发肿瘤和相应的转移淋巴结之间存在密切相关性。观察到的不完全一致性可以通过外在因素和癌细胞所在的微环境对基因表达的调节来解释。
This study investigated the changes, if any, in the level of expression of a well defined panel of cell proliferation, differentiation and apoptosis markers between the primary breast tumor and the corresponding synchronous lymph node metastasis from a population of patients with a comparable disease status, in terms of clinical features, and natural history. Ninety pure invasive ductal carcinomas with 10 or more axillary lymph nodes involved and without evidence of distant metastasis were included in this study. Primary tumor and corresponding metastatic lymph node tissue specimens were evaluated for the expression of Cyclin B1, MMP1 metalloproteinase, ICAM-1, RAR beta, Ki67, ER, PgR, p53, bcl-2 and c-erbB2 by immunohistochemistry using standard methods. The bivariate Pearson correlation analysis demonstrated a close relationship between primary and matching corresponding metastatic node. A high grade of correlation has been maintained even when staining results where categorized as positive/negative according to each one marker cut-off level of staining expression. We report the most extensive immunohistochemical analysis of biological determinants in a well defined population of patients with invasive ductal carcinomas and involvement of 10 or more axillary nodes and no distant metastasis. We found a close correlation between the primary tumor and corresponding metastatic node in terms of the expression of all 10 of the markers investigated in this study. The not complete concordance observed could be explained by the gene expression modulation by extrinsic factors and by the microenvironment in which the cancer cells reside.