MEASUREMENT OF ALBUMIN PERMEABILITY ACROSS ENDOTHELIAL MONOLAYERS INVITRO
MEASUREMENT OF ALBUMIN PERMEABILITY ACROSS ENDOTHELIAL MONOLAYERS INVITRO
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DOI：
10.1152/jappl.1987.62.3.1076
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发表时间：
1987-03-01
影响因子：
3.3
通讯作者：
MALIK, AB
中科院分区：
文献类型：
--
作者：
COOPER, JA;DELVECCHIO, PJ;MALIK, AB
我们开发了一个实验系统来测量培养的内皮单层的通透性。125i -白蛋白通过培养的肺内皮细胞的光-腔通量表示为清除率等于透性-表面积积。在30分钟基线期清除率测量后，在管腔侧加入一种测试剂，在30分钟的实验期间重新测量清除率。在对照研究中，基线清除率为0.343 +-。0.017 .mu.l /分钟。在对滤膜和未搅拌层的扩散阻力进行校正后，计算得到内皮单层的渗透率为1.2倍。纯cm / s。当培养基为试验剂时，实验清除率与基线值不变。在腔室中加入4 mM油酸后，清除率为0.528 .+-。0.017 .mu。L /min与基线值0.330 +-。0.008 .mu。l/min （P < 0.05）。这种方法允许计算内皮渗透率与校正未搅拌层和使用每个单层作为自己的控制。
We have developed an experimental system to measure the permeability of the cultured endothelial monolayer. The luminal-to-abluminal flux of 125I-albumin across cultured pulmonary endothelium was expressed as a clearance rate equal to the permeability-surface area product. After clearance rate measurement for a 30-min base-line period, a test agent was added to the luminal side, and the clearance rate was remeasured during a 30-min experimental period. In control studies the base-line clearance rate was 0.343 .+-. 0.017 .mu.l/min. After correction for the diffusional resistances of the filter and unstirred layers, the calculated permeability of the endothelial monolayer was 1.2 .times. 10-5 cm/s. When culture medium was the test agent, the experimental clearance rate was unchanged from the base-line value. After addition of 4 mM oleic acid to the luminal chamber, the clearance rate was 0.528 .+-. 0.017 .mu.l/min compared with a base-line value of 0.330 .+-. 0.008 .mu.l/min (P < 0.005). This method allows the calculation of endothelial permeability with correction for unstirred layers and the use of each monolayer as its own control.