Optimising expression and extraction of recombinant proteins in plants.
Optimising expression and extraction of recombinant proteins in plants.
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DOI：
10.3389/fpls.2022.1074531
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
重组蛋白对于研究、工业和医学用途是至关重要的。许多表达底盘可用于重组蛋白生产，而细菌和哺乳动物细胞培养物是最广泛使用的，最近的发展已经定位转基因植物底盘作为可行的，往往是优先的选择。植物底盘易于以低成本维护，具有巨大的可扩展性，并且能够产生大量携带复杂翻译后修饰的蛋白质。一些蛋白质靶标，包括针对人类疾病的抗体和疫苗，已经在植物中成功生产，突出了植物底盘的巨大潜力。本文综述了利用植物生产重组蛋白的最新进展，并总结了利用植物作为重组蛋白表达系统时必须考虑的因素，重点是在遗传水平上优化重组蛋白的表达，以及随后的目标蛋白的提取和纯化，这可以导致蛋白质稳定性的实质性改善，产率和纯度。
Recombinant proteins are of paramount importance for research, industrial and medical use. Numerous expression chassis are available for recombinant protein production, and while bacterial and mammalian cell cultures are the most widely used, recent developments have positioned transgenic plant chassis as viable and often preferential options. Plant chassis are easily maintained at low cost, are hugely scalable, and capable of producing large quantities of protein bearing complex post-translational modification. Several protein targets, including antibodies and vaccines against human disease, have been successfully produced in plants, highlighting the significant potential of plant chassis. The aim of this review is to act as a guide to producing recombinant protein in plants, discussing recent progress in the field and summarising the factors that must be considered when utilising plants as recombinant protein expression systems, with a focus on optimising recombinant protein expression at the genetic level, and the subsequent extraction and purification of target proteins, which can lead to substantial improvements in protein stability, yield and purity.
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影响因子：
5.3
作者：
Joseph M;Ludevid MD;Torrent M;Rofidal V;Tauzin M;Rossignol M;Peltier JB
通讯作者：
Peltier JB
影响因子：
13.8
作者：
Buyel, Johannes F.;Fischer, Rainer
通讯作者：
Fischer, Rainer
影响因子：
3
作者：
Avesani, Linda;Merlin, Matilde;Pezzotti, Mario
通讯作者：
Pezzotti, Mario
影响因子：
3.7
作者：
Iribarren PA;Berazategui MA;Cazzulo JJ;Alvarez VE
通讯作者：
Alvarez VE
影响因子：
5.1
作者：
Boyle, Patrick C.;Schwizer, Simon;Martin, Gregory B.
通讯作者：
Martin, Gregory B.