Enhancement of methanogenesis by electric syntrophy with biogenic iron-sulfide minerals.
Enhancement of methanogenesis by electric syntrophy with biogenic iron-sulfide minerals.
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DOI：
10.1002/mbo3.647
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发表时间：
2019-03
期刊：
影响因子：
3.4
通讯作者：
Igarashi K
中科院分区：
文献类型：
--
作者：
Kato S;Igarashi K
最近的研究表明，化学异养细菌和产甲烷古细菌之间的种间电子转移可以通过流经导电铁氧化物的电流来调节，这一过程被称为电合成。在这项研究中，我们对稻田土壤中的产甲烷微生物群落进行了富集化实验，以确定生物硫化铁是否能够实现电合成。虽然单独补充亚铁氢氧化铁或硫酸盐抑制了甲烷生成，但同时补充亚铁氢氧化铁和硫酸盐可以促进甲烷生成。在硫酸盐存在下，亚铁水合物转变为黑色沉淀物，主要由结晶度较低的硫化铁组成。微生物群落分析表明，在添加亚铁和硫酸盐的浓缩培养中，产甲烷古生菌和铁还原细菌和硫酸盐还原细菌(分别为甲烷八叠球菌、地杆菌和硫酸盐堆积杆菌)占主导地位。添加一种针对产甲烷古菌的抑制剂降低了地杆菌的丰度，但不会降低脱硫菌的丰度，这表明地杆菌是通过与产甲烷古菌的共养作用获得能量的。尽管硫酸盐和氧化铁等电子受体化合物被认为可以抑制甲烷生成，但这项研究表明，硫酸盐和氧化铁的共存可以通过(半)导电硫化铁硫化物的生物矿化促进甲烷生成，从而使甲烷生成能够通过电合成而实现。
Recent studies have shown that interspecies electron transfer between chemoheterotrophic bacteria and methanogenic archaea can be mediated by electric currents flowing through conductive iron oxides, a process termed electric syntrophy. In this study, we conducted enrichment experiments with methanogenic microbial communities from rice paddy soil in the presence of ferrihydrite and/or sulfate to determine whether electric syntrophy could be enabled by biogenic iron sulfides. Although supplementation with either ferrihydrite or sulfate alone suppressed methanogenesis, supplementation with both ferrihydrite and sulfate enhanced methanogenesis. In the presence of sulfate, ferrihydrite was transformed into black precipitates consisting mainly of poorly crystalline iron sulfides. Microbial community analysis revealed that a methanogenic archaeon and iron‐ and sulfate‐reducing bacteria (Methanosarcina, Geobacter, and Desulfotomaculum, respectively) predominated in the enrichment culture supplemented with both ferrihydrite and sulfate. Addition of an inhibitor specific for methanogenic archaea decreased the abundance of Geobacter, but not Desulfotomaculum, indicating that Geobacter acquired energy via syntrophic interaction with methanogenic archaea. Although electron acceptor compounds such as sulfate and iron oxides have been thought to suppress methanogenesis, this study revealed that coexistence of sulfate and iron oxide can promote methanogenesis by biomineralization of (semi)conductive iron sulfides that enable methanogenesis via electric syntrophy.
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