Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus
Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus
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DOI：
10.1073/pnas.092569199
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发表时间：
2002-05-14
影响因子：
11.1
通讯作者：
Meinke, A
中科院分区：
文献类型：
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作者：
Etz, H;Minh, DB;Meinke, A
对于设计有效的亚单位疫苗，识别感染病原体的患者群体免疫识别的所有抗原是至关重要的。我们已经开发了一种快速而有效的程序来识别这些公认的抗原，在这里我们提供了一种重要的人类病原体金黄色葡萄球菌的体内全面的抗原谱。通过与两种外膜蛋白(Lamb和FhuA)的融合，将金黄色葡萄球菌多肽展示在大肠杆菌表面，并与选择的抗体效价和调理活性较高的血清进行检测。共鉴定出60个抗原蛋白，其中大部分位于或预测位于细菌表面或分泌。这些抗原的鉴定及其与患者和健康个体血清的反应性极大地促进了选择有希望的候选疫苗进行进一步评估。这种方法利用了全基因组序列信息，有可能极大地加速和促进新疫苗的研制，并适用于任何在人类和/或实验动物中诱导抗体的病原体。
For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a rapid and efficient procedure to identify such commonly recognized antigens, and here we provide a comprehensive in vivo antigenic profile of Staphylococcus aureus, an important human pathogen. S. aureus peptides were displayed on the surface of Escherichia coli via fusion to one of two outer membrane proteins (LamB and FhuA) and probed with sera selected for high Ab titer and opsonic activity. A total of 60 antigenic proteins were identified, most of which are located or predicted to be located on the surface of the bacterium or secreted. The identification of these antigens and their reactivity with individual sera from patients and healthy individuals greatly facilitate the selection of promising vaccine candidates for further evaluation. This approach, which makes use of whole genome sequence information, has the potential to greatly accelerate and facilitate the formulation of novel vaccines and is applicable to any pathogen that induces Abs in humans and/or experimental animals.