BRUCELLOSIS OF CAMELS IN KUWAIT
BRUCELLOSIS OF CAMELS IN KUWAIT
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DOI：
10.1016/0147-9571(89)90002-7
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发表时间：
1989-01-01
影响因子：
2
通讯作者：
ELKHALADI, A
中科院分区：
文献类型：
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作者：
ALKHALAF, S;ELKHALADI, A
本研究调查了从科威特骆驼获得的血清和牛奶中是否存在布鲁氏菌抗体。还使用标准技术（Webridge Lab Techniques）从胎儿中分离布鲁氏菌菌株。采用三种血清学试验。这些试验为虎红平板试验（RBPT）、血清管凝集试验（SAT）和补体结合试验（CFT）。RBPT用于所有血清样品，SAT和CFT用于阳性RBPT。在SAT中显示效价为1：40或在CFT中显示效价为1：5或更高的骆驼被认为是阳性的。我们无法从Sedemine和牛奶奶油中分离出布鲁氏菌，但我们从胎儿布鲁氏菌中分离出了布鲁氏菌，并得到了英国Webridge实验室的确认。牛种布氏杆菌（Brucella abortus，生物变型1）。CFT法和RBPT法的阳性率为14.8%，SAT法的阳性率为10.8%。牛奶的患病率为8.0%。从5例胎儿中分离出2株流产布鲁氏菌。
This study investigated the presence of Brucella antibodies in serum and milk obtained from camels in Kuwait. Brucella strains were also isolated from the foetus using standard technique (Webridge Lab Techniques). Three serological tests for serum were adopted. These tests were Rose Bengal Plate Test (RBPT), the Serum Tube Agglutination Test (SAT) and the Complement Fixation Test (CFT). The RBPT was used for all sera samples, and both SAT and CFT were used for positive RBPT. Camels that showed a titer of 1:40 in SAT or 1:5 in CFT or greater were considered positive Thirteen of the samples examined were found positive by CFT (at 1:5); by SAT, they showed a were considered positive reactors but 1 + was considered suspicious. We were unable to isolate the Brucella organism from Sedemine and Cream of milk, but we isolated them from Foetus Brucella abortus and it is confirmed by Webridge Laboratory, U.K. It is Brucella abortus (Biovar 1). The prevalence rate was 14.8% from serum by the CFT and RBPT methods and 10.8% by the SAT method. For milk, the prevalence rate was 8.0%. Two Brucella abortus were isolated from 5 foetuses.