INTERPRETATION OF THE RESONANCE RAMAN-SPECTRUM OF BATHORHODOPSIN BASED ON VISUAL PIGMENT ANALOGS
INTERPRETATION OF THE RESONANCE RAMAN-SPECTRUM OF BATHORHODOPSIN BASED ON VISUAL PIGMENT ANALOGS
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DOI：
10.1021/bi00552a020
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
LUGTENBURG, J
中科院分区：
文献类型：
--
作者：
EYRING, G;CURRY, B;LUGTENBURG, J
视色素类似物的共振拉曼光谱用于评估红视紫红质中视网膜发色团结构的各种模型。 β-离子环上的18-甲基或多烯链上的19-甲基的氘化或去除表明，红视紫红质在853、875和920cm-1处的3个强低波数带不是由于外亚甲基或环模式造成的。将这些线分配给链上的平面外乙烯基氢运动可以最好地解释实验数据。计算表明，这些振动的强度可以用 10-30 度的扭曲来解释。关于链单键。 1100-1400 cm-1 拉曼指纹表明双键的构型是反式的。这表明红视紫红质发色团的结构是扭曲的全反式。
Resonance Raman spectra of visual pigment analogs were used to evaluate various models for the structure of the retinal chromophore in bathorhodopsin. Deuteration or removal of the 18-methyl on the .beta.-ionyl ring or of the 19-methyl on the polyene chain demonstrates that the 3 intense low wavenumber bands of bathorhodopsin at 853, 875 and 920 cm-1 are not due to exomethylene or ring modes. Assignment of these lines to out-of-plane vinyl hydrogen motions on the chain best accounts for the experimental data. Calculations show that the intensity of these vibrations can be explained by twists of 10-30.degree. about chain single bonds. The 1100-1400 cm-1 Raman fingerprint indicates that the configuration of the double bonds is trans. This suggests that the structure of the bathorhodopsin chromophore is twisted all-trans.