An STS-based radiation hybrid map of the human genome
An STS-based radiation hybrid map of the human genome
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DOI：
10.1101/gr.7.5.422
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发表时间：
1997-05-01
期刊：
影响因子：
7
通讯作者：
Cox, DR
中科院分区：
文献类型：
--
作者：
Stewart, EA;McKusick, KB;Cox, DR
我们使用一组83个全基因组辐射杂交体（斯坦福大学G3组），结合来自随机基因组DNA序列、先前绘制的遗传标记和表达序列的10，478个序列标记位点（STS），构建了人类基因组的物理图谱。在这些STS中，5049个是落入1766个高置信区间的框架标记。另外945个STS在它们的地图位置中与一个或多个框架标记物不可区分。这5994个映射的STS具有500kb的平均间隔。另外4484个STS相对于框架市场定位。将该地图上标记的顺序与从独立减数分裂和YAC STS含量地图中推导出的顺序进行比较，表明在定义高置信度箱时很少出现错误
We have constructed a physical map of the human genome by using a panel of 83 whole genome radiation hybrids (the Stanford G3 panel) in conjunction with 10,478 sequence-tagged sites (STSs) derived from random genomic DNA sequences, previously mapped genetic markers, and expressed sequences. Of these STSs, 5049 are framework markers that fall into 1766 high-confidence bins. An additional 945 STSs are indistinguishable in their map location from one or more of the framework markers. These 5994 mapped STSs have an average spacing of 500 kb. An additional 4484 STSs are positioned with respect to the framework markets. Comparison of the orders of markers oil this map with orders derived fi-om independent meiotic and YAC STS-content maps indicates that the error rare in defining high-confidence bins is