Expression of sex steroid hormone-related genes in the embryo of the leopard gecko
Expression of sex steroid hormone-related genes in the embryo of the leopard gecko
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DOI：
10.1016/j.ygcen.2007.04.010
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发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Park, Min Kyun
中科院分区：
文献类型：
--
作者：
Endo, Daisuke;Kanaho, Yoh-Ichiro;Park, Min Kyun
已知性类固醇激素在脊椎动物性别决定和分化中发挥核心作用。然而，在发育过程中，特别是在性腺性别决定期间，它们产生或接受的组织却很少被研究。在本研究中，我们鉴定了豹纹壁虎的 cDNA 序列，包括胆固醇侧链裂解酶 (P450scc) 编码区的全长；一种性别决定取决于温度的蜥蜴。随后对两种类固醇生成酶 P450scc 和 P450 芳香酶 (P450arom) 以及四种性类固醇激素受体、雄激素受体、雌激素受体 α 和 β 以及黄体酮受体进行胚胎表达分析。在性别决定的温度敏感期之前，在大脑和性腺中观察到两种类固醇生成酶的 mRNA 表达。在所有检查阶段的大脑和性腺中也检测到了四种性类固醇激素受体的 mRNA。这些结果表明发育中的豹纹壁虎大脑中存在不依赖性腺的性类固醇激素信号系统。 (C) 2007 Elsevier Inc. 保留所有权利。
Sex steroid hormones are known to play a central role in vertebrate sex determination and differentiation. However, the tissues in which they are produced or received during development, especially around the period of sex determination of the gonads, have rarely been investigated. In this study, we identified the cDNA sequence, including the full-length of the coding region of cholesterol side-chain cleavage enzyme (P450scc), from the leopard gecko; a lizard with temperature-dependent sex determination. Embryonic expression analysis of two steroidogenic enzymes, P450scc and P450 aromatase (P450arom), and four sex steroid hormone receptors, androgen receptor, estrogen receptor alpha and beta, and progesterone receptor, was subsequently conducted. mRNA expression of both steroidogenic enzymes was observed in the brain and gonads prior to the temperature-sensitive period of sex determination. The mRNAs of the four sex steroid hormone receptors were also detected in the brain and gonads at all stages examined. These results suggest the existence of a gonad-independent sex steroid hormone signaling system in the developing leopard gecko brain. (C) 2007 Elsevier Inc. All rights reserved.