Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases
Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases
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DOI：
10.1101/gad.10.15.1904
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发表时间：
1996-08-01
影响因子：
10.5
通讯作者：
Arndt, KT
中科院分区：
文献类型：
--
作者：
DiComo, CJ;Arndt, KT
我们确定了一个重要的酿酒酵母蛋白，Tap 42，与Sit 4，2A型相关的蛋白磷酸酶，并与2A型磷酸酶催化亚基。Tap 42与磷酸酶的结合不需要先前鉴定的磷酸酶亚基。遗传分析表明，Tap 42与两种磷酸酶都有积极的功能。TAP 42的突变可以赋予几乎完全的雷帕霉素抗性。此外，Tap 42/Sit 4和Tap 42/PP 2A复合物的形成受到营养生长信号和雷帕霉素敏感的Tor信号通路的调节。这些发现，结合在非允许的温度下的翻译缺陷的tap 42 -11突变体，表明Tap 42，Sit 4和PP 2A的Tot信号通路的组件。
We identified an essential Saccharomyces cerevisiae protein, Tap42, that associates with Sit4, a type 2A-related protein phosphatase, and with the type 2A phosphatase catalytic subunits. The association of Tap42 with the phosphatases does not require the previously identified phosphatase subunits. Genetic analysis suggests that Tap42 functions positively with both phosphatases. Mutations in TAP42 can confer almost complete rapamycin resistance. In addition, Tap42/Sit4 and Tap42/PP2A complex formation is regulated by nutrient growth signals and the rapamycin-sensitive Tor signaling pathway. These findings, combined with the defect in translation of the tap42-11 mutant at the nonpermissive temperature, suggest that Tap42, Sit4, and PP2A are components of the Tot signaling pathway.