Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila
Dopamine Receptor Dop1R2 Stabilizes Appetitive Olfactory Memory through the Raf/MAPK Pathway in Drosophila
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DOI：
10.1523/jneurosci.1572-19.2020
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发表时间：
2020-04-01
影响因子：
5.3
通讯作者：
Tanimoto, Hiromu
中科院分区：
文献类型：
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作者：
Sun, Huan;Nishioka, Tomoki;Tanimoto, Hiromu
在果蝇中，多巴胺信号传递到蘑菇体内在神经元，凯尼恩细胞（KC），是稳定嗅觉记忆的关键。关于记忆稳定的下游细胞内分子信号传导知之甚少。在这里，我们解决这个问题的背景下，糖奖励的嗅觉长期记忆（LTM）。我们发现，联合训练增加磷酸化的MAPK在KC，通过Dop 1 R2信号。一致地，KC中Dop 1 R2、Raf或MAPK表达的减弱选择性地损害LTM，但不损害短期记忆。此外，我们发现，通过在KC中表达活性Raf，可以挽救由Dop 1 R2敲低引起的LTM缺陷。因此，Dop 1 R2/Raf/MAPK通路是稳定食欲嗅觉记忆的多巴胺信号传导的关键下游效应器。
In Drosophila, dopamine signaling to the mushroom body intrinsic neurons, Kenyon cells (KCs), is critical to stabilize olfactory memory. Little is known about the downstream intracellular molecular signaling underlying memory stabilization. Here we address this question in the context of sugar-rewarded olfactory long-term memory (LTM). We show that associative training increases the phosphorylation of MAPK in KCs, via Dop1R2 signaling. Consistently, the attenuation of Dop1R2, Raf, or MAPK expression in KCs selectively impairs LTM, but not short-term memory. Moreover, we show that the LTM deficit caused by the knockdown of Dop1R2 can be rescued by expressing active Raf in KCs. Thus, the Dop1R2/Raf/MAPK pathway is a pivotal downstream effector of dopamine signaling for stabilizing appetitive olfactory memory.