Differential Expression of LaminB1 in the Developing Rat Cochlea
Differential Expression of LaminB1 in the Developing Rat Cochlea
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LaminB1 在发育中的大鼠耳蜗中的差异表达
DOI：
10.5152/iao.2019.6573
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发表时间：
2019-04-01
影响因子：
1
通讯作者：
Chu, Hanqi
中科院分区：
文献类型：
--
作者：
Du, Zhihui;Chen, Jin;Chu, Hanqi
目的：目的：探讨LaminB 1在出生后大鼠耳蜗中的表达规律，以及LaminB 1与耳蜗发育的关系。材料与方法：SD大鼠，出生后0（p0）~ 21（p21）天。分别解剖了血管纹（STV）的螺旋韧带、螺旋神经节细胞（SGC）和基底膜（BM）等组织，包括Corti器。结果：LaminB 1在耳蜗外毛细胞、内毛细胞、Kolliker's器、Reissner's膜、SGC、STV和螺旋韧带中均有表达。在耳蜗发育过程中，中阶周围的染色强度下降。LaminB 1 mRNA和蛋白在STV、SGC和BM中的表达在p0时达到最高水平，p21时逐渐下降至最低水平。结论：LaminB 1在耳蜗发育过程中有表达，并在耳蜗组织中受到发育调控，提示LaminB 1可能在耳蜗发育中起作用。本研究结果为进一步研究LaminB 1在外周听觉系统中的生理功能提供了理论依据。
OBJECTIVES: To explore the temporal expression pattern of LaminB1 in the cochlea of postnatal rat, and whether LaminB1 is associated with cochlear development.MATERIALS AND METHODS: Sprague-Dawley rats ranging from postnatal day 0 (p0) to 21 (p21) were used. The tissues of stria vascularis (STV) including spiral ligament, spiral ganglion cell (SGC), and basilar membrane (BM), including the organ of Corti, were dissected, respectively. Immunofluorescence, quantitative real-time polymerase chain reaction, and western blot were applied to detect the expression of LaminB1 in individual cochlear tissues at both mRNA and protein levels.RESULTS: Immunofluorescence revealed that LaminB1 was localized in the outer hair cells, inner hair cells, Kolliker's organ, Reissner's membrane, SGC, STV, and spiral ligament. The intensity of staining surrounding the scala media decreased during cochlear development. The expression of LaminB1 mRNA and protein in STV, SGC, and BM was at a maximum level at p0 but gradually declined to a minimum level at p21.CONCLUSION: Our research provided direct evidence that LaminB1 was expressed in the developing cochlea and developmentally regulated in cochlear tissues, suggesting a possible role of LaminB1 in cochlear development. Our result provided a theoretical basis for further study about the physiological function of LaminB1 in the peripheral auditory system.