Antisense oligodeoxynucleotide complexation with phosphoryl-choline diblock copolymers and its effect on cell transfection.
Antisense oligodeoxynucleotide complexation with phosphoryl-choline diblock copolymers and its effect on cell transfection.
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DOI：
10.1016/j.jconrel.2011.08.070
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发表时间：
2011-11
期刊：
影响因子：
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通讯作者：
Tiantian Sun;Zhuoqi Zhang;Xichuan Cao;Min Zhang-;Xia Zhao;Qi Zhang;Minmin Chen
中科院分区：
文献类型：
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作者：
Tiantian Sun;Zhuoqi Zhang;Xichuan Cao;Min Zhang-;Xia Zhao;Qi Zhang;Minmin Chen
在不同的N/P比例下形成MPC 30-DEA 70/c-myc AS-ODN复合物，并将其转染HeLa细胞。采用凝胶电泳、荧光显微镜、流式细胞术和MTT法对复合物进行了研究。结果表明，新型阳离子磷酰胆碱共聚物MPC 30-DEA 70可负载和转运人c-myc反义寡核苷酸。随着N/P比的增加，复合物的转染效率和细胞生长抑制作用均显著增强。
MPC30-DEA70/c-myc AS-ODN complexes were formed at different N/P ratios and transferred into HeLa cells. The complexes were studied using gel electrophoresis, fluorescent microscopy, FCM, and MTT assay. It was shown that the novel cationic phosphorylcholine copolymer MPC30–DEA70could load and transport human c-myc antisense oligonucleotides. The transfection efficiency of the complexes and cell growth inhibition were significantly enhanced with increasing of N/P ratios.