The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa.
The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa.
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抗菌光动力疗法对人纤毛呼吸道粘膜的影响。
DOI：
10.1002/lary.23502
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Loebel,Nicolas
中科院分区：
文献类型：
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作者：
Biel,MerrillA;Jones,JohnW;Pedigo,Lisa;Gibbs,Aaron;Loebel,Nicolas
慢性复发性鼻窦炎（CRS）是美国最常见的慢性疾病之一.有一个重要的CRS患者亚群，尽管有严格的治疗方案，包括手术，过敏治疗和长期抗生素治疗，但仍然对治愈有抵抗力。抗菌光动力疗法（aPDT）是一种非侵入性、非抗生素、广谱抗菌的治疗方法。我们先前的体外研究表明，aPDT在单次治疗后使CRS多微生物生物膜和嗜酸性细菌和真菌减少> 99.9%。然而，在人类治疗之前，必须证明aPDT治疗不会导致鼻窦纤毛呼吸上皮的组织学损伤。本研究的目的是证明aPDT治疗在活体人纤毛呼吸道粘膜模型（EpiAirway™）上的安全性。治疗组包括非治疗对照、单独激光、单独光敏剂和治疗性光敏剂和光组合（aPDT）。在处理完成时，将EpiAirway组织固定在10%福尔马林中，石蜡包埋，切片，H&E染色并封片。所有样品均设盲并由人类病理学家进行显微镜检查，以评估aPDT对组织、纤毛或粘膜腺体的任何影响。结果与治疗parameters.ResultsThe EpiAirway组织学研究表明，在任何治疗groups.ConclusionsaPDT的呼吸道纤毛或粘膜上皮细胞的组织学改变是一个安全的治疗CRS导致没有组织学改变的人纤毛呼吸道粘膜是在人类鼻窦。喉镜，2012年
BackgroundChronic recurrent sinusitis (CRS) is one of the most common chronic conditions in the United States. There is a significant subpopulation of CRS patients who remain resistant to cure despite rigorous treatment regimens including surgery, allergy therapy, and prolonged antibiotic therapy. Antimicrobial photodynamic therapy (aPDT) is a noninvasive nonantibiotic broad spectrum antimicrobial treatment. Our previous in vitro studies demonstrated that aPDT reduced CRS polymicrobial biofilm and planktonic bacteria and fungi by > 99.9% after a single treatment. Prior to human treatment however, aPDT treatment must be demonstrated to not result in histologic damage to the sinus ciliated respiratory epithelium. The objective of this study was to demonstrate the safety of aPDT treatment on a living human ciliated respiratory mucosal model (EpiAirway™).MethodsA study of aPDT treatment of EpiAirway was performed. Treatment groups included a nontreatment control, laser light alone, photosensitizer alone, and therapeutic photosensitizer and light combination (aPDT). At completion of treatment, the EpiAirway tissue was fixed in 10% formalin, paraffin‐embedded, sectioned, H&E stained and mounted. All samples were blinded and microscopically examined by a human pathologist to assess any effect of aPDT on the tissue, cilia, or mucosal glands. The results were correlated with the treatment parameters.ResultsThe EpiAirway histologic study demonstrated no histologic alteration of the respiratory cilia or mucosal epithelium in any of the treatment groups.ConclusionsaPDT is a safe treatment for CRS resulting in no histologic alteration of human ciliated respiratory mucosa as is found in the human sinuses. Laryngoscope, 2012