Diurnal rhythms in the incorporation and secretion of 3H-proline and 3H-galactose by cartilage cells and osteoblasts in various bone-forming sites in growing rats
Diurnal rhythms in the incorporation and secretion of 3H-proline and 3H-galactose by cartilage cells and osteoblasts in various bone-forming sites in growing rats
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DOI：
10.1016/j.odw.2012.09.001
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发表时间：
2013-03-01
影响因子：
0.4
通讯作者：
Shinoda, Hisashi
中科院分区：
文献类型：
--
作者：
Igarashi, Kaoru;Saeki, Shuichi;Shinoda, Hisashi
目的：昼夜变化是骨生理的基本特征。然而，这一现象的特点在骨领域尚未完全了解。本研究的目的是通过h -3-脯氨酸和h -3-半乳糖放射自显影研究生长大鼠不同骨形成部位软骨细胞和成骨细胞基质合成和分泌活性的日变化。材料与方法：雄性Wistar大鼠适应12/12 h光照/黑暗周期2周后，分为6组，分别于00:00、04:00、08:00、12:00、16:00、20:00注射示踪剂。注射1小时后处死动物，取股骨、下颌骨和颅骨，进行放射自显影处理。对注射h -3-脯氨酸的动物进行股骨骨骺、蝶枕关节联合和矢状面缝合检查。对注射3h -半乳糖的动物进行股骨骨骺和下颌髁检查。结果：在所有检测区域，两种细胞类型的银颗粒总数和基质/细胞比在24小时内变化显著，呈现出基本相同的日波动模式，在13:00达到峰值，在01:00达到最低点。这些昼夜节律显示出相当大的偏移，范围从138.5%到240.6%。结论：我们的研究结果清楚地表明，生长大鼠各骨形成部位软骨细胞和成骨细胞的基质合成和分泌活动遵循几乎相同的昼夜节律，在光明期中期达到峰值，在黑暗期中期达到最低点。(C) 2012爱思唯尔有限公司和日本正畸学会。版权所有。
Purpose: Diurnal variation is an essential feature of bone physiology. However, characteristics of this phenomenon were not fully understood in the bone field. The purpose of this study was to investigate diurnal variations in the matrix-synthetic and secretory activities of cartilage cells and osteoblasts in various bone-forming sites in growing rats by radioautography with H-3-proline and H-3-galactose.Materials and methods: After acclimatization to a 12/12 h light/dark cycle for 2 weeks, male Wistar rats were divided into six groups and injected with the tracers at 00:00, 04:00, 08:00, 12:00, 16:00 or 20:00, respectively. One hour after the injection, the animals were sacrificed, and femora, mandibles and skull were removed and processed for radioautography. Femoral epiphysis, spheno-occipital synchondrosis and sagittal suture were examined for the H-3-proline- injected animals. Femoral epiphysis and mandibular condyle were examined for the 3H-galactose-injected animals.Results: In all area examined, both total number of silver grains and matrix/cell ratio for both cell types varied significantly over a 24-h period, showing essentially the same pattern of diurnal fluctuation with a peak at 13:00 and a nadir at 01:00. These diurnal rhythms revealed considerable excursions ranging from 138.5% to 240.6%.Conclusion: Our results clearly demonstrated that in growing rats, the matrix-synthetic and secretory activities of cartilage cells and osteoblasts in various bone-forming sites followed almost the same diurnal rhythm with a peak in the middle of the light period and a nadir in the middle of the dark period. (C) 2012 Elsevier Ltd and the Japanese Orthodontic Society. All rights reserved.