Preserving the viscous coral surface mucus layer using low-acid glycol methacrylate (GMA) resin
Preserving the viscous coral surface mucus layer using low-acid glycol methacrylate (GMA) resin
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DOI：
10.1007/s00338-019-01791-y
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发表时间：
2019-06-01
期刊：
影响因子：
3.5
通讯作者：
Bythell, John C.
中科院分区：
文献类型：
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作者：
Guppy, Reia;Brown, Barbara;Bythell, John C.
表面粘液层(SML)在珊瑚的健康、疾病和应激反应中至关重要；然而，可视化完整的SML是具有挑战性的。组织学准备过程中的脱水会导致粘液凝胶层的收缩和变形，而脆弱的、未附着的粘液渗出物通常会完全消失。在这里，我们描述了一种新的技术，使用水溶性乙二醇甲基丙烯酸酯树脂包埋，更准确地保存原位SML。保存下来的SML的厚度与之前使用玻璃微探头在活珊瑚上测量的厚度相似。这项技术允许对SML结构进行微观可视化，以及厚度和连续性测量，这是SML在健康和疾病中功能的重要指标。
The surface mucus layer (SML) is of critical importance in health, disease, and stress responses of corals; however, visualising the intact SML is challenging. Dehydration during histological preparation causes shrinkage and deformation of the mucus gel layer, while fragile, unattached mucus exudates are typically lost altogether. Here, we describe a novel technique using water-soluble glycol methacrylate resin embedding that more accurately preserves the in situ SML. Thickness of the preserved SML is similar to that previously measured on live corals using a glass microprobe. The technique allows microscopic visualisation of the SML structure, as well as thickness and continuity measurements, which are important indicators of SML function in health and disease.