grim, a novel cell death gene in Drosophila
grim, a novel cell death gene in Drosophila
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DOI：
10.1101/gad.10.14.1773
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发表时间：
1996-07-15
影响因子：
10.5
通讯作者：
Abrams, JM
中科院分区：
文献类型：
--
作者：
Chen, P;Nordstrom, W;Abrams, JM
果蝇程序性细胞死亡需要75C1，2的基因组间隔。我们发现了一个新的凋亡激活因子grim，它映射了两个以前在该区域发现的细胞死亡基因reaper（rpr）和head involution deficient（hid）。Grim RNA的表达与胚胎发育各个阶段的程序性细胞死亡发生一致，而异位诱导Grim在转基因动物和细胞培养中引发了广泛的细胞凋亡。grim对细胞的杀伤作用被p35的共表达所阻断，p35是一种病毒产物，可以使ICE样蛋白酶失活，并且不需要rpr或hid的功能。预测的grim蛋白与rpr共享一个共同的氨基末端基序。然而，至少在一种情况下，grim足以引起细胞凋亡，而rpr不能。因此，这种可怕的基因产物可能在细胞死亡信号的平行回路中发挥作用，最终激活一组常见的下游凋亡效应物。
A genomic interval at 75C1,2 is required for programmed cell death in Drosophila. We identified a new activator of apoptosis, grim, which maps between two previously identified cell death genes in this region reaper (rpr) and head involution defective (hid). Expression of grim RNA coincided with the onset of programmed cell death at all stages of embryonic development, whereas ectopic induction of grim triggered extensive apoptosis in both transgenic animals and in cell culture. Cell killing by grim was blocked by coexpression of p35, a viral product that inactivates ICE-like proteases, and did not require the functions of rpr or hid. The predicted grim protein shares an amino-terminal motif in common with rpr. However, grim was sufficient to elicit apoptosis in at least one context, where rpr was not. The grim gene product might thus function in a parallel circuit of cell death signaling that ultimately activates a common set of downstream apoptotic effecters.