Expression of vascular endothelial growth factor (VEGF) in various compartments of the human hair follicle
Expression of vascular endothelial growth factor (VEGF) in various compartments of the human hair follicle
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DOI：
10.1007/s004030050370
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发表时间：
1998-12-01
影响因子：
3
通讯作者：
Orfanos, CE
中科院分区：
文献类型：
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作者：
Kozlowska, U;Blume-Peytavi, U;Orfanos, CE
毛囊血管化似乎与毛发周期调节过程密切相关，其中涉及生长因子、细胞因子和其他生物活性分子。特别是，血管内皮生长因子(VEGF)对血管生成和血管通透性至关重要，可能负责在生长期维持毛囊周围的适当血管形成。本研究的目的是比较不同毛囊室来源的不同培养细胞在体外的血管生成能力，即血管内皮生长因子基因的稳定表达。体外培养人毛乳头细胞(DPC)、纤维鞘成纤维细胞、真皮成纤维细胞、毛囊和毛囊间角质形成细胞，用RT-PCR方法检测VEGF在mRNA水平的表达，用放射免疫沉淀法和免疫细胞化学法检测VEGF蛋白的合成。用免疫组织化学方法检测人毛囊末端组织中血管内皮生长因子的表达。在本报告中，首次描述了四种不同的血管生成能力不同的VEGF分子亚型在不同类型的卵泡细胞中的表达。培养的卵泡细胞强烈表达四种血管内皮生长因子分子的mRNA，它们分别是121、145、165和189个氨基酸剪接变异体，其中最突出的是121个氨基酸分子。DPC和其他间充质细胞如纤维鞘成纤维细胞和真皮成纤维细胞在体内和体外均强表达VEGF mRNA，合成46 kDa的VEGF蛋白，而毛囊和毛囊间角质形成细胞在体外表达的VEGF mRNA和蛋白水平低于间充质细胞。由于血管内皮生长因子在DPC中的表达最高，我们认为DPC主要参与可能与人类毛发周期有关的血管生成过程。
Hair follicle vascularization appears to be closely related to the processes involved in hair cycle regulation, in which growth factors, cytokines and other bioactive molecules are involved. In particular, vascular endothelial growth factor (VEGF), essential for angiogenesis and vascular permeability, may be responsible for maintaining proper vasculature around the hair follicle during the anagen growth phase. The aim of our study was to compare the in vitro angiogenic capacity, i.e. the steady-state expression of the VEGF gene, of different cultured cell types derived from normal human hair follicles, corresponding to different follicular compartments. Human dermal papilla cells (DPC), fibrous sheath fibroblasts, dermal fibroblasts, and follicular and interfollicular keratinocytes were cultured and studied in vitro for VEGF expression at the mRNA level using RT-PCR, and for VEGF protein synthesis by radioimmunoprecipitation and immunocytochemistry. In vivo examination for VEGF expression in human terminal hair follicles was performed using immunohistochemical methods. In the present report the expression of four different VEGF molecular isoforms, differing in their angiogenic capacity, are described in different cultured follicular cell types for the first time. Cultured follicular cells strongly expressed mRNA of four VEGF molecular species identified as the 121-, 145-, 165- and 189-amino acid splice variants, the most prominent being the 121-amino acid molecule. DPC, and also other mesenchymal cells such as fibrous sheath fibroblasts and dermal fibroblasts, in vivo and in vitro strongly expressed VEGF mRNA and synthesized a 46-kDa VEGF protein, whereas follicular and interfollicular keratinocytes in vitro expressed lower levels of VEGF mRNA and proteins than mesenchymal cells. As the highest expression of VEGF was found in DPC, we suggest that DPC are mainly responsible for angiogenic processes possibly related to the human hair cycle.