STRUCTURE-INDEPENDENT NUCLEOTIDE-SEQUENCE ANALYSIS
STRUCTURE-INDEPENDENT NUCLEOTIDE-SEQUENCE ANALYSIS
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DOI：
10.1073/pnas.76.5.2232
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
KRAMER, FR
中科院分区：
文献类型：
--
作者：
MILLS, DR;KRAMER, FR
在为 RNA 测序而合成的核酸片段中用肌苷取代鸟苷可有效防止电泳分析过程中二级结构的形成。因此，测序凝胶中每个片段的迁移率严格取决于其分子量。肌苷取代应显着提高 DNA 和 RNA 测序中获得的分辨率。
Substitution of inosine for guanosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its MW. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.