A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.
A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.
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DOI：
10.1186/1477-5956-10-39
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发表时间：
2012-06-08
期刊：
影响因子：
2
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Jia X;Yin L;Feng Y;Peng X;Ma F;Yao Y;Liu X;Zhang Z;Yuan Z;Zhang L
酒精性损伤已成为肝硬化的主要原因之一。然而，乙醇诱导的损伤的分子机制尚未完全了解。为此，我们对大鼠模型进行了动态质膜蛋白质组学研究。建立了大鼠肝炎肝硬化模型。用蔗糖密度梯度离心法纯化肝纤维化2期和4期（S2和S4）肝组织的质膜。其纯化通过蛋白质印迹进行验证。采用双向电泳（2DE）技术分离质膜蛋白，并通过串联质谱技术鉴定差异表达蛋白。经质谱分析，从S2 ~ S4共鉴定出16个一致的差异表达蛋白。Western blotting检测差异表达蛋白annexin A6和annexin A3的表达，免疫组化检测annexin A3的表达。我们的研究提示了乙醇改变蛋白质表达以促进肝纤维化进展的可能机制。这些差异表达蛋白可能成为治疗酒精性肝硬化的新的药物靶点。
Alcohol-induced injury has become one of the major causes for liver cirrhosis. However, the molecular mechanisms of ethanol-induced injury are not fully understood. To this end, we performed a dynamic plasma membrane proteomic research on rat model. A rat model from hepatitis to liver cirrhosis was developed. Plasma membrane from liver tissue with liver fibrosis stage of 2 and 4 (S2 and S4) was purified by sucrose density gradient centrifugation. Its purification was verified by western blotting. Proteins from plasma membrane were separated by two-dimensional electrophoresis (2DE) and differentially expressed proteins were identified by tandem mass spectrometry. 16 consistent differentially expressed proteins from S2 to S4 were identified by mass spectrometry. The expression of differentially expressed proteins annexin A6 and annexin A3 were verified by western blotting, and annexin A3 was futher verified by immunohistochemistry. Our research suggests a possible mechanism by which ethanol alters protein expression to enhance the liver fibrosis progression. These differentially expressed proteins might be new drug targets for treating alcoholic liver cirrhosis.
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