Activated macrophages are responsible for the tumor‐inhibitory effect in mice receiving intravenous injection of OK‐432
Activated macrophages are responsible for the tumor‐inhibitory effect in mice receiving intravenous injection of OK‐432
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活化的巨噬细胞负责静脉注射 OK-432 的小鼠的肿瘤抑制作用
DOI：
10.1002/ijc.2910330217
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发表时间：
1984
影响因子：
6.4
通讯作者：
N. Ishida
中科院分区：
文献类型：
--
作者：
M. Saito;M. Nanjo;Etsuko Aonuma;T. Noda;I. Nakadate;T. Ebina;N. Ishida
Mouse spleen cells, either pretreated in vitro with 100 U/ml of OK‐432‐induced IFN gamma for 18 h or obtained from mice 24 or 48 h after i.v. injection of OK‐432(100 μg/mouse), were examined for their anti‐tumor effect by Winn's neutralization assay against Meth‐A tumor cells in BALB/c mice. Spleen cells treated in vitro or obtained in vivo 24 h after i.v. injection clearly neutralized the growth of admixed Meth‐A cells. Two booster injections of 200 U of IFN gamma near the tumor site accelerated this neutralizating effect. In order to determine the effector subpopulation, inhibitory spleen cells were treated with either anti‐Thy‐1 monoclonal antibody plus complement, antiasialo GM1 serum plus complement or with adherence on plastic plates followed by Sephadex G‐10 column treatment, The effector cell activity in Winn assay was lost only after the removal of macrophages through plastic plate adherence and Sephadex G‐10 column treatment, but not after anti‐Thy‐1 or anti‐asialo GM1 treatment, with either in vitro‐ or in vivo‐treated spleen‐cell populations. The growth of Meth‐A cells was inhibited not only by these activated macrophages in Winn's assay, but also by adoptive transfer of OK‐432‐induced cytotoxic macrophages intralesionally 4 days after the implantation of 1 × 106 Meth‐A cells. Our evidence suggests that the systemic action of OK‐432 can be explained by the effect of induced. IFNgamma, through the activation of macrophages.
小棒状杆菌在腹膜巨噬细胞激活中的作用。
影响因子：
4.3
作者：
Chapes,SK;Haskill,S
通讯作者：
Haskill,S