Solution Structure of the Histidine-Containing Phosphocarrier Protein from Staphylococcus carnosus
Solution Structure of the Histidine-Containing Phosphocarrier Protein from Staphylococcus carnosus
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肉葡萄球菌含组氨酸磷酸载体蛋白的溶液结构
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Kalbitzer
中科院分区：
文献类型：
--
作者：
A. Görler;W. Hengstenberg;M. Kravanja;W. Beneicke;T. Maurer;H. Kalbitzer
本文用二维和三维核磁共振（NMR）技术研究了肉葡萄球菌（Staphylococcus carnosus）含组氨酸磷酸化载体蛋白的溶液结构。其主要结构单元是一个反平行的β-折叠片，具有四股A、B、C和D，按拓扑结构A-D-B-C排列。链A包含残基2至8，链B包含残基32至37，链C包含残基40至43，链D包含残基59至66。三个右手螺旋排列在β-折叠片层的顶部。阻碍a从残基16到达29，螺旋B从残基48到达53，螺旋c从残基72到达83。β-折叠片的链B和C通过II型转弯连接。Ser-31的羟基质子与溶剂的交换非常缓慢，以至于可以在基于同核J-耦合的二维NMR光谱中检测到交叉峰。活性中心His-15的咪唑环，其在pH 7.2下确定的结构中部分带电，位于螺旋a的N-末端上方，垂直于其轴。His-15的Nδ1原子接受来自酶I的磷酰基，暴露于溶剂。
The solution structure of histidine-containing phosphocarrier protein fromStaphylococcus carnosus was determined by two- and three-dimensional nuclear magnetic resonance (NMR) spectroscopy on uniformly15N-enriched protein. The main structural element is an antiparallel β-pleated sheet with four strands A, B, C, and D arranged with the topology A-D-B-C. Strand A comprises residues 2 to 8, strand B residues 32 to 37, strand C reidues 40 to 43, and strand D residues 59 to 66. Three right-handed helices are arranged on top of the β-pleated sheet. Helix a reaches from residue 16 to 29, helix b from residue 48 to 53, and helix c from residue 72 to 83. Strands B and C of the β-pleated sheet are connected by a type II turn. The hydroxyl proton of Ser-31 is exchanging with the solvent so slowly that cross peaks can be detected in two-dimensional NMR spectra based on homonuclearJ-couplings. The imidazole ring of the active-center His-15, which is partly charged in the structure determined at pH 7.2, is located above the N-terminal end of helix a, perpendicular to its axis. The Nδ1 atom of His-15, accepting the phosphoryl from enzyme I, is exposed to the solvent.
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Beneski,DA;Nakazawa,A;Weigel,N;Hartman,PE;Roseman,S
通讯作者：
Roseman,S
DOI：
10.1073/pnas.82.4.1074
发表时间：
1985-01-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
通过同核和异核核磁共振波谱重新检查大肠杆菌含组氨酸蛋白质的二级和三级结构。
影响因子：
2.9
作者：
Hammen,PK;Waygood,EB;Klevit,RE
通讯作者：
Klevit,RE
来自大肠杆菌的含组氨酸蛋白质的二维 1H NMR 研究。
影响因子：
2.9
作者：
Klevit,RE;Waygood,EB
通讯作者：
Waygood,EB