Cost-Effective Production of Surfactin from Xylose-Rich Corncob Hydrolysate Using Bacillus subtilis BS-37
Cost-Effective Production of Surfactin from Xylose-Rich Corncob Hydrolysate Using Bacillus subtilis BS-37
复制标题
使用枯草芽孢杆菌 BS-37 从富含木糖的玉米芯水解产物中经济高效地生产表面活性剂
DOI：
10.1007/s12649-017-0052-5
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发表时间：
2019-02-01
影响因子：
3.2
通讯作者：
Li, Shuang
中科院分区：
文献类型：
--
作者：
Chen, Chen;Lin, Junzhang;Li, Shuang
目的玉米芯水解物、羽毛水解物废料和味精厂废料是从农业废弃物中提取的廉价原料。以富含木糖的玉米芯水解液和富含有机氮的低成本残渣为原料，优化枯草芽孢杆菌BS-37发酵生产表面活性素的工艺条件。用高效液相色谱法测定了玉米芯水解液的糖组成和表面活性素效价。结果筛选出一株高效表面活性素产生菌B。枯草芽孢杆菌BS-37可以利用木糖作为唯一碳源，但添加有机氮源是生产表面活性素的必要条件。因此，利用富含木糖的玉米芯水解液和富含有机氮的低成本残渣来生产表面活性素。菌株BS-37能够耐受显着浓度的几种抑制性化合物中发现的玉米芯水解产物，其中乙酸甚至增强表面活性素的生产。此外，NaOH-中和的玉米芯水解液是更适合于表面活性素生产比传统的Ca（OH）2-中和的feeds.ConclusionsWe实现了最大的523 mg/L的表面活性素产量从NaOH-中和的玉米芯水解液和羽毛水解液废料。此外，产物含有55.3%的最期望的表面活性素同种型C15。据我们所知，这是第一次报道以木糖为主要发酵碳源生产表面活性素。
PurposeCorncob hydrolysate, feather hydrolysate waste (FHW) and glutamate mill waste (GMW) are inexpensive raw materials derived from agricultural waste biomass. The aim of the study was to optimize the production of surfactin from the xylose-rich corncob hydrolysate and low-cost residues rich in organic nitrogen using Bacillus subtilis BS-37.MethodsHydrolysis of corncob was carried out with 1.5% H2SO4, FHW or GMW was used as nitrogen source to produce surfactin. Sugar compositions of corncob hydrolysate and surfactin titer were determined by HPLC. The dry weight method was used to measure biomass in fermentation broth.ResultsWe discovered that the efficient surfactin producer B. subtilis BS-37 can use xylose as sole carbon source, but the addition of organic nitrogen sources was essential for surfactin production. Consequently, the xylose-rich corncob hydrolysate and low-cost residues rich in organic nitrogen were used to produce surfactin. Strain BS-37 was able to tolerate significant concentrations of several inhibitory compounds found in corncob hydrolysate, whereby acetic acid even enhanced surfactin production. Furthermore, NaOH-neutralized corncob hydrolysate was more suitable for surfactin production than the traditional Ca(OH)2-neutralized feedstock.ConclusionsWe achieved a maximal surfactin yield of 523 mg/L from NaOH-neutralized corncob hydrolysate and feather hydrolysate waste. Furthermore, the product contained 55.3% of the most desirable surfactin isoform C15. To our best knowledge, this is the first report of surfactin production with xylose as the main fermentable carbon source.