Stabilized polyglycolic acid fibre based tubes for tissue engineering
Stabilized polyglycolic acid fibre based tubes for tissue engineering
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DOI：
10.1016/0142-9612(96)85756-5
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发表时间：
1996-01-01
期刊：
影响因子：
14
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Mooney, DT;Mazzoni, CL;Langer, R
聚乙醇酸（PGA）纤维网是移植细胞的有吸引力的候选者，但它们不能抵抗显著的压缩力。为了稳定PGA补片，将溶解在氯仿中的聚（L-乳酸）（PLLA）和聚（D，L-乳酸-共-乙醇酸）（PLGA）的50/50共聚物的雾化溶液喷洒在形成为中空管的补片上。PLLA和PLGA涂覆PGA纤维并物理结合相邻的纤维。粘合的图案和程度由雾化溶液中聚合物的浓度和喷涂在装置上的聚合物的总质量控制。器械的抗压缩性随着粘接程度的增加而增加，PLLA粘接管比PLGA粘接管抵抗更大的压缩力。与PLLA粘合的管比与PLGA粘合的装置降解更慢。将PLLA粘合管植入大鼠体内表明，该装置在纤维血管组织向内生长期间保持其结构，导致形成具有中心腔的管状结构。这些装置工程化特定组织的潜力通过以下发现来展示：在体外接种到装置上的平滑肌细胞和内皮细胞形成具有适当细胞分布的管状组织。
Polyglycolic acid (PGA) fibre meshes are attractive candidates to transplant cells, but they are incapable of resisting significant compressional forces. To stabilize PGA meshes, atomized solutions of poly(L-lactic acid) (PLLA) and a 50/50 copolymer of poly(D,L-lactic-co-glycolic acid) (PLGA) dissolved in chloroform were sprayed over meshes formed into hollow tubes. The PLLA and PLGA coated the PGA fibres and physically bonded adjacent fibres. The pattern and extent of bonding was controlled by the concentration of polymer in the atomized solution and the total mass of polymer sprayed on the device. The compression resistance of devices increased with the extent of bonding, and PLLA bonded tubes resisted larger compressive forces than PLGA bonded tubes. Tubes bonded with PLLA degraded more slowly than devices bonded with PLGA. Implantation of PLLA bonded tubes into rats revealed that the devices maintained their structure during fibrovascular tissue ingrowth, resulting in the formation of a tubular structure with a central lumen. The potential of these devices to engineer specific tissues was exhibited by the finding that smooth muscle cells and endothelial cells seeded onto devices in vitro formed a tubular tissue with appropriate cell distribution.