A rhodamine–quinoline based chemodosimeter capable of recognising endogenous OCl− in human blood cells
A rhodamine–quinoline based chemodosimeter capable of recognising endogenous OCl− in human blood cells
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DOI：
10.1039/c4ra03200d
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发表时间：
2014-06
期刊：
影响因子：
3.9
通讯作者：
S. Goswami;Sangita Das;Krishnendu Aich;P. K. Nandi;Kakali Ghoshal;C. Quah;M. Bhattacharyya;H. Fun;H. Abdel‐Aziz
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文献类型：
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作者：
S. Goswami;Sangita Das;Krishnendu Aich;P. K. Nandi;Kakali Ghoshal;C. Quah;M. Bhattacharyya;H. Fun;H. Abdel‐Aziz
本文设计、合成并表征了一种罗丹明-喹啉化学剂量计（RHQ）。通过单晶X射线研究证实了传感器的结构。它在其他研究的分析物中选择性地检测次氯酸盐（OCl−）。在加入OCl−后，它显示出比色和橙红色荧光“开启”。在水-乙腈（1/1，v/v）介质中，RHQ中罗丹明螺内酰胺环的OCl−促进开环引起较大的吸光度和荧光增强，对其他竞争性分析物无显著响应。此外，我们在这里证明，RHQ可以内源性检测人血细胞（外周血单核细胞）中的OCl−。它在“浸棒”法中也表现出优异的性能。通过密度泛函理论计算得到了探针的优化结构。此外，探针的检测极限在10 - 8 M范围内。
A rhodamine–quinoline based chemodosimeter (RHQ) has been designed, synthesized and characterized in this paper. The structure of the sensor is confirmed through single crystal X-ray study. It detects hypochlorite (OCl−) selectively among other analytes studied. It showed colorimetric and orange-red fluorescence “turn-on” upon addition of OCl−. The OCl−-promoted ring opening of the rhodamine spirolactam ring in RHQ evokes a large absorbance as well as fluorescence enhancement in water–acetonitrile (1/1, v/v) medium with no significant response to other competitive analytes. Furthermore, we demonstrate here that RHQ can endogenously detect OCl− in human blood cells (peripheral blood mononuclear cells). It also exhibits excellent performance in the “dip stick” method. The optimized structure of the probe is calculated by density functional theory calculations. Moreover, the limit of detection of the probe is in the 10−8 M range.