Analysis of the incidence of infectious pancreatic necrosis mortality in pedigreed Atlantic salmon, Salmo salar L., populations
Analysis of the incidence of infectious pancreatic necrosis mortality in pedigreed Atlantic salmon, Salmo salar L., populations
复制标题
DOI：
10.1111/j.1365-2761.2006.00758.x
复制
发表时间：
2006-11-01
影响因子：
2.5
通讯作者：
Woolliams, J. A.
中科院分区：
文献类型：
--
作者：
Guy, D. R.;Bishop, S. C.;Woolliams, J. A.
共有 77 124 条大西洋鲑鱼幼崽，代表 197 个全同胞家庭，由 149 只雄性和 197 只雌性繁殖，在转移到三个不同的海水地点后，经历了传染性胰腺坏死病毒 (IPNV) 的现场挑战。首次 IPN 死亡发生在转移后 45 天，不同地点的流行持续时间在 37 至 92 天之间。通过 PIT（被动集成应答器）标签记录和 DNA 基因分型可追踪死亡者的父母家庭。计算每个地点每个家庭的全同胞家庭平均死亡率发生率。根据卡方的异质性，使用半同胞家庭内的发病率和全同胞家庭内的发病率方差来估计遗传力，两者均在观察到的潜在责任量表上进行。观察到的跨地点家庭之间的相关性用于估计遗传相关性。总体死亡率为10.8%，不同地点之间差异很小，为10.3%至11.9%。研究发现，责任量表的遗传力为中等到强，范围在 0.24 到 0.81 之间，汇总估计值为 0.43，高于通常与疾病特征相关的遗传力。位点之间的遗传相关性都很显着，在 0.71 到 0.78 之间，这表明位点内显示的遗传变异的一个重要组成部分是所有位点所共有的。结果表明，田间挑战可以产生关于抗性家族差异的非常好的遗传信息，特别是在多个位点复制时，这些信息随后可用于选择对 IPN 具有更强抗性的大西洋鲑鱼育种品系。
A total of 77 124 Atlantic salmon post-smolts, representing 197 full-sib families produced by 149 males and 197 females, experienced a field challenge from infectious pancreatic necrosis virus (IPNV), following transfer to three separate seawater sites. The first IPN mortality was observed 45 days after transfer, and the duration of the epidemic varied between 37 and 92 days among sites. Mortalities were traced to their parental families by PIT (Passive Integrated Transpondes) tag records and DNA genotyping. Full-sib family mean incidence of mortality was calculated for each family on each site. Heritabilities were estimated based on the heterogeneity of chi-square using incidence within half-sib families and the variance in incidence among full-sib families, both on the observed an underlying liability scale. The observed correlation among families across sites was used to estimate genetic correlations. The overall mortality rate was 10.8%, with only small differences between sites, ranging from 10.3% to 11.9%. Heritabilities on the liability scale were found to be moderate to strong, and ranged between 0.24 and 0.81, with a pooled estimate of 0.43, greater than is typically associate with disease traits. Genetic correlations among sites were all substantial, between 0.71 and 0.78, and indicated that a substantial component of the genetic variation displayed within sites was common to all. The results show that field challenges can yield very good genetic information on family differences in resistance, especially when replicated over sites, which may then be developed for use in selection for breeding strains of Atlantic salmon with greater resistance to IPN.