IMMUNOASSAY FOR THE DETECTION AND QUANTITATION OF INFECTIOUS HUMAN RETROVIRUS, LYMPHADENOPATHY-ASSOCIATED VIRUS (LAV)
IMMUNOASSAY FOR THE DETECTION AND QUANTITATION OF INFECTIOUS HUMAN RETROVIRUS, LYMPHADENOPATHY-ASSOCIATED VIRUS (LAV)
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用于检测和定量传染性人类逆转录病毒淋巴腺病相关病毒（LAV）的免疫测定法
DOI：
10.1016/0022-1759(85)90489-2
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
MARTIN, LS
中科院分区：
文献类型：
--
作者：
MCDOUGAL, JS;CORT, SP;MARTIN, LS
描述了一种新的夹心酶联免疫分析 (ELISA)，用于检测 LAV 感染的人淋巴细胞培养物上清液中的人逆转录病毒、淋巴结病相关病毒 (LAV)。这种 LAV 捕获免疫测定法与逆转录酶测定法相比具有优势，尽管事实上它是在少 20 倍的上清液材料上进行的。由于该测定可以对 0.1 ml 培养物上清液进行，并且是通过 ELISA 方法完成的，因此可以非常方便地监测淋巴细胞培养物的 LAV 接种，并且可以进行感染性病毒的终点滴定（ID-50 测定）。演示了捕获测定和 ID-50 测定在消毒剂和血清中和实验中的应用。
A new sandwich enzyme-linked immunoassay (ELISA) for detecting the human retrovirus, lymphadenopathy-associated virus (LAV), in supernates of LAV-infected human lymphocytes cultures was described. This LAV capture immunoassay compares favorably with the reverse transcriptase assay, despite the fact that it is performed on 20-fold less supernate material. Because the assay can be performed on 0.1 ml of culture supernate and is done by an ELISA method, LAV inoculation of lymphocyte cultures can be monitored quite conveniently and endpoint titrations of infectious virus (ID-50 assays) can be performed. The application of the capture assay and ID-50 assay to disinfectant and serum neutralization experiments was demonstrated.