Localization of proteolytic activity in psoriatic skin.
Localization of proteolytic activity in psoriatic skin.
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DOI：
10.1111/j.1365-2133.1982.tb00398.x
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发表时间：
1982-11
影响因子：
10.3
通讯作者：
L. Dubertret;B. Bertaux;M. Fosse;R. Touraine
中科院分区：
文献类型：
--
作者：
L. Dubertret;B. Bertaux;M. Fosse;R. Touraine
将未固定的皮肤切片在37° C下在Hanks培养基中孵育1小时，允许蛋白水解活性在银屑病病变的角质形成细胞上层中表达，并且在某些情况下，在银屑病患者的未受累皮肤中表达，但在正常皮肤中从未表达。这种蛋白水解被DFP和TLCK抑制，这是先前描述为在银屑病病变中增加的中性蛋白酶的已知抑制剂。这种蛋白水解活性的地形分析表明，人表皮中蛋白酶-抗蛋白酶平衡的紊乱可能与银屑病病变的出现有关。
Incubation of unfixed slices of skin for 1 hat 37° C in Hanks' medium allowed the expression of proteolytic activity in the upper keratinocyte layers in psoriatic lesions and, in some cases, in uninvolved skin of psoriatics, but never in normal skin. This proteolysis was inhibited by DFP and TLCK, known inhibitors of the neutral proteinase previously described as increased in psoriatic lesions. The topographical analysis of this proteolytic activity suggests that a disturbance of the protease‐antiprotease equilibrium in human epidermis may be implicated in the appearance of psoriatic lesions.