Polyunsaturated linolenoyl-CoA modulates ERF-VII-mediated hypoxia signaling in Arabidopsis
Polyunsaturated linolenoyl-CoA modulates ERF-VII-mediated hypoxia signaling in Arabidopsis
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DOI：
10.1111/jipb.12875
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发表时间：
2020-01-29
影响因子：
11.4
通讯作者：
Xiao, Shi
中科院分区：
文献类型：
--
作者：
Zhou, Ying;Tan, Wei-Juan;Xiao, Shi
在植物中，洪水造成的淹没会导致缺氧，这会损害能量生产并影响植物生长，生产力和生存。在拟南芥中，缺氧诱导与AP2.12相关的VII组乙烯应答转录因子（RAP 2.12）的核定位，随后其与质膜锚定的酰基辅酶A结合蛋白1（ACBP 1）和ACBP 2解离。在这里，我们表明，多不饱和亚麻酰辅酶A（18：3-CoA）调节RAP 2.12从质膜释放。浸没导致18：3-CoA显著增加，但18：0-、18：1-和18：2-CoA显著降低。18：3-CoA的应用促进了绿色荧光蛋白（GFP）融合体RAP 2.12-GFP、低氧应答ERF 1-GFP和RAP 2.3-GFP的核积累，并提高了低氧应答基因的转录水平。ACBP 1和ACBP 2降低的植物（acbp 1 ACBP 2-RNAi，由ACBP 2 RNA干扰acbp 1突变体产生）对缺氧的耐受性降低，并损害了18：3-CoA诱导的缺氧相关基因的表达。在长链酰基辅酶A合成酶2（LACS 2）和脂肪酸去饱和酶3（FAD 3）的敲除突变体和过表达系中，酰基辅酶A池的大小和18：3-CoA水平与ERF-VII介导的信号传导和缺氧耐受性密切相关。这些发现表明，长链酰基辅酶A的多不饱和功能的重要机制，在调节植物缺氧信号，通过调节ACBP-ERF-VII动力学。
In plants, submergence from flooding causes hypoxia, which impairs energy production and affects plant growth, productivity, and survival. In Arabidopsis, hypoxia induces nuclear localization of the group VII ethylene-responsive transcription factor RELATED TO AP2.12 (RAP2.12), following its dissociation from the plasma membrane-anchored ACYL-COA BINDING PROTEIN1 (ACBP1) and ACBP2. Here, we show that polyunsaturated linolenoyl-CoA (18:3-CoA) regulates RAP2.12 release from the plasma membrane. Submergence caused a significant increase in 18:3-CoA, but a significant decrease in 18:0-, 18:1-, and 18:2-CoA. Application of 18:3-CoA promoted nuclear accumulation of the green fluorescent protein (GFP) fusions RAP2.12-GFP, HYPOXIA-RESPONSIVE ERF1-GFP, and RAP2.3-GFP, and enhanced transcript levels of hypoxia-responsive genes. Plants with decreased ACBP1 and ACBP2 (acbp1 ACBP2-RNAi, produced by ACBP2 RNA interference in the acbp1 mutant) had reduced tolerance to hypoxia and impaired 18:3-CoA-induced expression of hypoxia-related genes. In knockout mutants and overexpression lines of LONG-CHAIN ACYL-COA SYNTHASE2 (LACS2) and FATTY ACID DESATURASE 3 (FAD3), the acyl-CoA pool size and 18:3-CoA levels were closely related to ERF-VII-mediated signaling and hypoxia tolerance. These findings demonstrate that polyunsaturation of long-chain acyl-CoAs functions as important mechanism in the regulation of plant hypoxia signaling, by modulating ACBP-ERF-VII dynamics.