Asymmetry in semicircular canal diameters may account for circling behavior in EphB-deficient mice.
Asymmetry in semicircular canal diameters may account for circling behavior in EphB-deficient mice.
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半规管直径的不对称可能是 EphB 缺陷小鼠的转圈行为的原因。
DOI：
10.1002/lary.24506
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Henkemeyer,Mark
中科院分区：
文献类型：
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作者：
Lee,KennethH;Lee,James;Shao,Dongmei;Dravis,Christopher;Henkemeyer,Mark
目的/假设确定上级半规管大小的差异是否解释了表型行为，表明EphB缺陷小鼠的前庭病变。在已知具有摇头和转圈行为的纯合EphB 2和EphB 3双敲除小鼠中分析右侧和左侧上级SCC直径的差异。结果进行了比较，在野生型对照，显示没有迹象vestibulopathy.MethodsAxial冷冻切片通过上级（SCC）进行了分析，通过光学显微镜和直径的左，右运河测量μm的EphB 2和EphB 3双敲除小鼠，以及在野生型对照小鼠。结果EphB 2和EphB 3双基因敲除小鼠的上级SCC直径小于野生型（109.0 ± 21.4 μm vs. 185.0 ± 5.2 μm，P< 0.0001）。EphB 2/EphB 3双敲除小鼠的上级SCC的左右直径的平均差异为29.0 ± 8.7 μm;在野生型对照中，该差异为6.0 ± 5.1 μm（P= 0.002）。此外，盘旋的方向似乎是独立的偏侧的较小（或较大）的上级SCC.ConclusionMice缺乏EphB 2/EphB 3信号转导有较小的上级SCC和不对称的管腔大小之间的左侧和右侧。较大与较小的偏侧性与盘旋行为的方向无关。证据等级N/A.Laryngoscope，124：E278-E282，2014
Objectives/HypothesisDetermine if differences in right and left semicircular size account for phenotypic behavior, indicating vestibulopathy in EphB deficient mice.Study DesignThe diameters of the superior semicircular canals (SCC) were measured. The differences in the right and left superior SCC diameters were analyzed in homozygous EphB2 and EphB3 double knockout mice known to have head bobbing and circling behavior. Results were compared to similar analysis in wild type controls that displayed no signs of vestibulopathy.MethodsAxial frozen sections through the superior (SCC) were analyzed by light microscopy; and the diameters of the left and right canals were measured in μm for both EphB2 and EphB3 double knockout mice, as well as in wild type control mice. The differences in diameter between the left and right superior SCC was determined for each animal.ResultsOverall, the EphB2 and EphB3 double knockout mice had smaller superior SCC diameters compared to wild type (109.0 ± 21.4 μm vs. 185.0 ± 5.2 μm (P< 0.0001). The mean difference in left and right diameter of the superior SCC of EphB2/EphB3 double knockout mice was 29.0 ± 8.7 μm; in wild‐type controls this difference was 6.0 ± 5.1 μm (P= 0.002). In addition, the direction of circling appeared to be independent of the laterality of the smaller (or larger) superior SCC.ConclusionMice deficient in EphB2/EphB3 signaling have smaller superior SCC and asymmetry in lumen sizes between the left and right sides. The laterality of the larger versus smaller is not correlated with the direction of circling behavior.Level of EvidenceN/A.Laryngoscope, 124:E278–E282, 2014