UPTAKE KINETICS AND INTRACELLULAR-LOCALIZATION OF HYPOCRELLIN PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY - A CONFOCAL MICROSCOPY STUDY
UPTAKE KINETICS AND INTRACELLULAR-LOCALIZATION OF HYPOCRELLIN PHOTOSENSITIZERS FOR PHOTODYNAMIC THERAPY - A CONFOCAL MICROSCOPY STUDY
复制标题
DOI：
10.1111/j.1751-1097.1995.tb09880.x
复制
发表时间：
1995-06-01
影响因子：
3.3
通讯作者：
MCPHEE, MS
中科院分区：
文献类型：
--
作者：
MILLER, GG;BROWN, K;MCPHEE, MS
竹红菌素是在生物系统中具有光敏特性的天然存在的化合物。我们合成了竹红菌素B的衍生物，这些衍生物在光动力疗法的临床应用中有望作为光敏剂。在EMT 6/艾德小鼠肿瘤细胞单层培养中，用荧光共聚焦显微镜半定量测定竹红菌素B和几种竹红菌素同系物的细胞内定位和摄取动力学。每种化合物具有独特的摄取动力学。尽管迄今为止没有测试的化合物证明了核标记，但大多数化合物可以在溶酶体、高尔基体、内质网中检测到，并且在较小程度上可以在细胞膜中检测到。没有两种化合物给出相同的标记分布。假设差异源于每种化合物的物理化学特性特征，这可能最终影响光毒性的主要形式。
Hypocrellins are naturally occurring compounds with photosensitizing properties in biological systems. We have prepared synthetic derivatives of hypocrellin B, which have promise as photosensitizers in the clinical application of photodynamic therapy, The intracellular localization and uptake kinetics of hypocrellin B and several selected hypocrellin congeners were determined semiquantitatively by fluorescence confocal microscopy in monolayer cultures of EMT6/Ed murine tumor cells. Each compound had unique uptake kinetics. Although no compound tested to date has demonstrated nuclear labeling, most could be detected in lysosomes, Golgi, endoplasmic reticulum and, to a minor extent, in cellular membranes. No two compounds gave identical labeling distributions. The differences are assumed to originate in physicochemical properties characteristic of each compound, which may ultimately impact upon the primary modality of phototoxicity.