PAX-3, A NOVEL MURINE DNA-BINDING PROTEIN EXPRESSED DURING EARLY NEUROGENESIS
PAX-3, A NOVEL MURINE DNA-BINDING PROTEIN EXPRESSED DURING EARLY NEUROGENESIS
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DOI：
10.1002/j.1460-2075.1991.tb08054.x
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发表时间：
1991-05-01
期刊：
影响因子：
11.4
通讯作者：
GRUSS, P
中科院分区：
文献类型：
--
作者：
GOULDING, MD;CHALEPAKIS, G;GRUSS, P
我们描述了Pax-3，一种新的小鼠配对盒基因表达专门在胚胎发育过程中的分离和表征。 Pax-3基因编码479个氨基酸，M（r）为56 kd，含有一个配对结构域和一个配对型同源结构域。 Pax-3蛋白是一种DNA结合蛋白，特异性识别果蝇偶跳基因上游的e5序列。 Pax-3转录本首先在8.5天的小鼠胚胎中检测到，其中它们仅限于神经上皮的背侧部分和相邻的分段的真皮肌节。 在早期神经发生期间，Pax-3表达仅限于发育中脊髓室区的有丝分裂细胞以及后脑、中脑和间脑的不同区域。在10-12天的胚胎中，Pax-3的表达也见于发育中的脊神经节的神经嵴细胞、颅面中外胚层以及10和11天胚胎的肢体间充质中。
We describe the isolation and characterization of Pax-3, a novel murine paired box gene expressed exclusively during embryogenesis. Pax-3 encodes a 479 amino acid protein with an M(r) of 56 kd containing both a paired domain and a paired-type homeodomain. The Pax-3 protein is a DNA binding protein that specifically recognizes the e5 sequence present upstream of the Drosophila even-skipped gene. Pax-3 transcripts are first detected in 8.5 day mouse embryos where they are restricted to the dorsal part of the neuroepithelium and to the adjacent segmented dermomyotome. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme of 10 and 11 day embryos.