Vitronectin is significant in the adhesion of lens epithelial cells to PMMA polymers
Vitronectin is significant in the adhesion of lens epithelial cells to PMMA polymers
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DOI：
10.1002/jbm.a.30017
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发表时间：
2004-06-01
影响因子：
4.9
通讯作者：
Migonney, W
中科院分区：
文献类型：
--
作者：
Evans, MDM;Pavon-Djavid, G;Migonney, W
眼内透镜手术的主要并发症是由透镜上皮细胞的再生长引起的视力下降（继发性白内障）。聚甲基丙烯酸甲酯（PMMA）是一种常用的人工透镜材料。本研究探讨了透镜上皮细胞与PMMA和已知可抑制细胞增殖的功能化PMMA基粘附剂的初始粘附机制。将兔透镜上皮细胞在含有去除纤连蛋白或玻连蛋白（或两者）的血清的培养基中在测试聚合物表面上培养，以鉴定这些蛋白在细胞粘附的初始过程中的作用。60分钟后对粘附细胞进行定量，并在两种聚合物的每个血清处理中比较肌动蛋白细胞骨架和焦点接触形成。玻连蛋白是显着更有效的初始细胞附着到两种聚合物比纤连蛋白。PMMA上的正常细胞扩散需要玻连蛋白，并且独立于纤连蛋白，而PMMA上的细胞扩散是异常的，并且需要同时存在纤连蛋白和玻连蛋白。总之，这些结果有助于解释先前在官能化PMMA上显示的细胞增殖的抑制。这项工作有助于设计用于人工晶状体的聚合物，抑制靶细胞的增殖。(C)2004 Wiley Periodicals，Inc.
A major complication of intraocular lens surgery is diminished visual acuity caused by the regrowth of lens epithelial cells (secondary cataract). Poly methylmethacrylate (PMMA) is a commonly used intraocular lens material. This study addresses the mechanisms underlying the initial adhesion of lens epithelial cells to PMMA and a functionalized PMMA-based terpolymer known to inhibit cell proliferation. Rabbit lens epithelial cells were cultured on the test polymer surfaces in medium containing serum depleted of either fibronectin or vitronectin (or both) to identify the role of these proteins in the initial process of cell adhesion. Adherent cells were quantitated after 60 min, and the actin cytoskeleton and focal contact formation were compared in each serum treatment on both polymers. Vitronectin was significantly more effective for initial cell attachment to both polymers than fibronectin. Normal cell spreading on PMMA required vitronectin and was independent of fibronectin, whereas cell spreading on the terpolymer was abnormal and required the presence of fibronectin and vitronectin together. Together, these results help to explain the inhibition of cell proliferation previously shown on the functionalized PMMA. This work contributes to the design of a polymer for use in intraocular lenses that inhibits proliferation of the target cells. (C) 2004 Wiley Periodicals, Inc.