VND-INTERACTING2 effectively inhibits transcriptional activities of VASCULAR-RELATED NAC-DOMAIN7 through a conserved sequence
VND-INTERACTING2 effectively inhibits transcriptional activities of VASCULAR-RELATED NAC-DOMAIN7 through a conserved sequence
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DOI：
10.5511/plantbiotechnology.22.0122a
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发表时间：
2022-06-01
影响因子：
1.6
通讯作者：
Yamaguchi, Masatoshi
中科院分区：
文献类型：
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作者：
Ailizati, Aili;Nagahage, Isura Sumeda Priyadarshana;Yamaguchi, Masatoshi
拟南芥 NAC 结构域转录因子 VND-INTERACTING2 (VNI2) 最初被分离为与另一个 NAC 结构域转录因子血管相关 NAC-DOMAIN7 (VND7) 的相互作用蛋白，VND7 是木质部导管元件分化的主要调节因子。 VNI2 通过形成蛋白复合物抑制 VND7 的转录激活活性。在这里，为了深入了解 VNI2 如何调节 VND7，我们尝试确定抑制 VND7 所需的 VNI2 氨基酸区域。 VNI2 的 C 末端氨基酸序列与乙烯响应元件结合因子 (ERF) 相关的两亲抑制 (EAR) 基序相似，在转录抑制子中保守。瞬时表达测定表明，VNI2 的 EAR 样基序并不是抑制 VND7 所必需的。 VNI2的C端缺失系列揭示了VNI2直向同源物中高度保守的10个氨基酸残基有助于有效抑制VND7的转录激活活性。对异位表达 VNI2 的转基因植物的观察表明，所鉴定的 10 个氨基酸序列强烈影响木质部导管的形成和植物生长。这些数据表明VNI2的10个氨基酸序列在其转录抑制活性和木质部导管形成的负调节中具有重要作用。
An Arabidopsis NAC domain transcription factor VND-INTERACTING2 (VNI2) was originally isolated as an interacting protein with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel element differentiation. VNI2 inhibits transcriptional activation activity of VND7 by forming a protein complex. Here, to obtain insights into how VNI2 regulates VND7, we tried to identify the amino acid region of VNI2 required for inhibition of VND7. VNI2 has an amino acid sequence similar to the ETHYLENERESPONSIVE ELEMENT BINDING FACTOR (ERF)-associated amphiphilic repression (EAR) motif, conserved in transcriptional repressors, at the C-terminus. A transient expression assay showed that the EAR-like motif of VNI2 was not required for inhibition of VND7. The C-terminal deletion series of VNI2 revealed that 10 amino acid residues, highly conserved in the VNI2 orthologs contributed to effective repression of the transcriptional activation activity of VND7. Observation of transgenic plants ectopically expressing VNI2 showed that the identified 10 amino acid sequence strongly affected xylem vessel formation and plant growth. These data indicated that the 10 amino acid sequence of VNI2 has an important role in its transcriptional repression activity and negative regulation of xylem vessel formation.