African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever
African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever
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DOI：
10.1099/jgv.0.000024
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发表时间：
2016-07-01
影响因子：
3.8
通讯作者：
Rock, D. L.
中科院分区：
文献类型：
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作者：
Burmakina, G.;Malogolovkin, A.;Rock, D. L.
非洲猪瘟（ASF）是全球养猪业面临的一种新兴疾病威胁。目前还没有可用的非洲猪瘟疫苗，而且由于缺乏对非洲猪瘟病毒（ASFV）毒株多样性程度和在猪体内起保护作用的病毒抗原的了解，进展受到阻碍。猪疫苗接种/攻毒实验的现有数据表明，非洲猪瘟保护性免疫是血液吸附抑制（HAI）血清型特异性的。需要更好地了解ASFV HAI血清学组及其性质的多样性，以及改进ASFV分离株血清型的方法。本研究表明，编码ASFV CD2v和c型凝集素蛋白的基因位点介导了HAI的血清学特异性，CD2v/ c型凝集素基因分型为ASFV按血清型分组提供了一种简单的方法，从而促进了ASFV菌株多样性的研究，并为最终的疫苗设计、开发和有效使用提供了必要的信息。非洲猪瘟（ASF）是全球养猪业面临的一种新兴疾病威胁。目前还没有可用的非洲猪瘟疫苗，而且由于缺乏对非洲猪瘟毒株多样性程度和在猪中具有类型特异性保护性免疫的病毒抗原的了解，进展受到阻碍。猪疫苗接种/攻毒实验的现有数据表明，非洲猪瘟保护性免疫可能是血液吸附抑制（HAI）血清型特异性的。最近，我们发现两种ASFV蛋白CD2v （EP402R）和c型凝集素（EP153R）是介导HAI血清学特异性的必要和充分条件（Malogolovkin et al., 2015）。在这里，我们使用ASFV跨血清型嵌合病毒和猪的疫苗/攻毒实验，证明血清型特异性CD2v和/或c型凝集素蛋白对抵抗同源ASFV感染是重要的。因此，这些病毒蛋白代表了重要的ASFV保护性抗原，应该作为未来疫苗设计和开发的目标。此外，这些数据支持HAI血清型特异性保护性免疫的概念。
African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASF virus (ASFV) strain diversity and the viral antigens responsible for protection in the pig. Available data from vaccination/challenge experiments in pigs indicate ASF protective immunity is haemadsorption inhibition (HAI) serotype-specific. A better understanding of ASFV HAI serological groups and their diversity in nature, as well as improved methods to serotype ASFV isolates, is needed. Here, we demonstrated that the genetic locus encoding ASFV CD2v and C-type lectin proteins mediates HAI serological specificity and that CD2v/C-type lectin genotyping provides a simple method to group ASFVs by serotype, thus facilitating study of ASFV strain diversity in nature, and providing information necessary for eventual vaccine design, development and efficacious use.African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASFV strain diversity and the viral antigens conferring type-specific protective immunity in pigs. Available data from vaccination/challenge experiments in pigs indicate that ASF protective immunity may be haemadsorption inhibition (HAI) serotype-specific. Recently, we have shown that two ASFV proteins, CD2v (EP402R) and C-type lectin (EP153R), are necessary and sufficient for mediating HAI serological specificity (Malogolovkin et al., 2015).. Here, using ASFV inter-serotypic chimeric viruses and vaccination/challenge experiments in pigs, we demonstrate that serotype-specific CD2v and/or C-type lectin proteins are important for protection against homologous ASFV infection. Thus, these viral proteins represent significant protective antigens for ASFV that should be targeted in future vaccine design and development. Additionally, these data support the concept of HAI serotype-specific protective immunity.