Neural crest stem cells from hair follicles and boundary cap have different effects on pancreatic islets in vitro
Neural crest stem cells from hair follicles and boundary cap have different effects on pancreatic islets in vitro
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DOI：
10.3109/00207454.2014.950373
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发表时间：
2015-07-01
影响因子：
2.2
通讯作者：
Kozlova, Elena N.
中科院分区：
文献类型：
--
作者：
Kosykh, Anastasiia;Ngarnjariyawat, Anongnad;Kozlova, Elena N.
目的：源自边界帽的神经嵴干细胞 (bNCSC) 在与胰岛共培养/移植后，在体外和体内显着促进产生胰岛素的 β 细胞的存活、增殖和功能 [1, 2]。最近，我们发现对 β 细胞的有益作用需要 bNCSC 和 β 细胞之间的钙粘蛋白接触 [3, 4]。在这里，我们研究了毛囊 (HF) NCSC（人类同种异体移植的潜在来源）是否对 β 细胞产生类似的积极作用。材料和方法：我们将表达增强型绿色荧光蛋白的小鼠的 HF-NCSC 或 bNCSC 与表达 DxRed 的小鼠或 NMRI 小鼠的胰岛共培养，并使用 qRT-PCR 分析和免疫组织化学比较它们向胰岛细胞的迁移和对 β 细胞增殖的影响以及 NCSC 和胰岛之间的细胞内关系。结果：尽管两种类型的 NCSC 在胰岛存在时都广泛迁移，但只有 bNCSC 表现出向胰岛的定向迁移，诱导 β 细胞增殖并增加 bNCSC 和 β 细胞之间连接处钙粘蛋白的存在。即使β细胞和HF-NCSC直接接触，也没有检测到钙粘蛋白表达。结论：这些观察结果表明 HF-NCSC 不会像 bNCSC 那样对 β 细胞产生相同的积极作用。此外，这些数据表明，HF-NCSC 诱导钙粘蛋白表达可能有助于它们在共培养中和移植后支持 β 细胞的能力。
Purpose: Neural crest stem cells derived from the boundary cap (bNCSCs), markedly promote survival, proliferation and function of insulin producing beta-cells in vitro and in vivo after coculture/transplantation with pancreatic islets [1, 2]. Recently, we have shown that beneficial effects on beta-cells require cadherin contacts between bNCSCs and beta-cells [3, 4]. Here we investigated whether hair follicle (HF) NCSCs, a potential source for human allogeneic transplantation, exert similar positive effects on beta-cells. Materials and Methods: We established cocultures of HF-NCSCs or bNCSCs from mice expressing enhanced green fluorescent protein together with pancreatic islets from DxRed expressing mice or NMRI mice and compared their migration towards islet cells and effect on proliferation of beta-cells as well as intracellular relations between NCSCs and islets using qRT-PCR analysis and immunohistochemistry. Results: Whereas both types of NCSCs migrated extensively in the presence of islets, only bNCSCs demonstrated directed migration toward islets, induced beta-cell proliferation and increased the presence of cadherin at the junctions between bNCSCs and beta-cells. Even in direct contact between beta-cells and HF-NCSCs, no cadherin expression was detected. Conclusions: These observations indicate that HF-NCSCs do not confer the same positive effect on beta-cells as demonstrated for bNCSCs. Furthermore, these data suggest that induction of cadherin expression by HF-NCSCs may be useful for their ability to support beta-cells in coculture and after transplantation.